Soil compaction is a major disturbance associated with logging, but we lack a fundamental understanding of how this affects the soil microbiome. We assessed the structural resistance and resilience of the microbiome using a high-throughput pyrosequencing approach in differently compacted soils at two forest sites and correlated these findings with changes in soil physical properties and functions. Alterations in soil porosity after compaction strongly limited the air and water conductivity. Compaction significantly reduced abundance, increased diversity, and persistently altered the structure of the microbiota. Fungi were less resistant and resilient than bacteria; clayey soils were less resistant and resilient than sandy soils. The strongest effects were observed in soils with unfavorable moisture conditions, where air and water conductivities dropped well below 10% of their initial value. Maximum impact was observed around 6-12 months after compaction, and microbial communities showed resilience in lightly but not in severely compacted soils 4 years post disturbance. Bacteria capable of anaerobic respiration, including sulfate, sulfur, and metal reducers of the Proteobacteria and Firmicutes, were significantly associated with compacted soils. Compaction detrimentally affected ectomycorrhizal species, whereas saprobic and parasitic fungi proportionally increased in compacted soils. Structural shifts in the microbiota were accompanied by significant changes in soil processes, resulting in reduced carbon dioxide, and increased methane and nitrous oxide emissions from compacted soils. This study demonstrates that physical soil disturbance during logging induces profound and long-lasting changes in the soil microbiome and associated soil functions, raising awareness regarding sustainable management of economically driven logging operations.

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Temperate forest soils are usually efficient sinks for the greenhouse gas methane, at least in the absence of significant amounts of methanogens. We demonstrate here that trafficking with heavy harvesting machines caused a large reduction in CH 4 consumption and even turned well-aerated forest soils into net methane sources. In addition to studying methane fluxes, we investigated the responses of methanogens after trafficking in two different forest sites. Trafficking generated wheel tracks with different impact (low, moderate, severe, and unaffected). We found that machine passes decreased the soils' macropore space and lowered hydraulic conductivities in wheel tracks. Severely compacted soils yielded high methanogenic abundance, as demonstrated by quantitative PCR analyses of methyl coenzyme M reductase (mcrA) genes, whereas these sequences were undetectable in unaffected soils. Even after a year after traffic compression, methanogen abundance in compacted soils did not decline, indicating a stability of methanogens here over time. Compacted wheel tracks exhibited a relatively constant community structure, since we found several persisting mcrA sequence types continuously present at all sampling times. Phylogenetic analysis revealed a rather large methanogen diversity in the compacted soil, and most mcrA gene sequences were mostly similar to known sequences from wetlands. The majority of mcrA gene sequences belonged either to the order Methanosarcinales or Methanomicrobiales, whereas both sites were dominated by members of the families Methanomicrobiaceae Fencluster, with similar sequences obtained from peatland environments. The results show that compacting wet forest soils by heavy machinery causes increases in methane production and release.

Elucidation of the mechanisms involved in long-distance water transport in trees requires knowledge of the water distribution within the sapwood and heartwood of the stem as well as of the earlywood and latewood of an annual ring. X-ray computed tomography is a powerful tool for measuring density distributions and water contents in the xylem with high spatial resolution. Ten-to 20-year-old spruce (Picea abies L. KARST.) and oak (Quercus robur) trees grown in the field were used throughout the experiments. Stem and branch discs were collected from different tree heights, immediately deep frozen, and used for the tomographic determinations of spatial water distributions. Results are presented for single-tree individuals, demonstrating heartwood and sapwood distribution throughout their entire length as well as the water relations in single annual rings of both types of wood. Tree rings of the sapwood show steep water gradients from latewood to earlywood, whereas those of the heartwood reflect water deficiency in both species. Although only the latest two annual rings of the ringporous species are generally assumed to transport water, we found similar amounts of water and no tyloses in all rings of the oak sapwood, which indicates that at least water storage is important in the whole sapwood.The aim of this study was to test high-resolution computed tomography (CT) for water relations in spruce (Picea abies L. KARST.) and oak (Quercus robur) stems rather then to analyze the general features of tree conductive systems that are already well documented (Hartig, 1855; Mac Dougal, 1925; C ermak et al., 1992).The CT method was developed by Cormack and Hounsfield and today has become a standard examination method in the medical field and material sciences (Hounsfield, 1980). In plants, CT has been shown to be capable of measuring the inner properties of wood. For instance, studies of CT scanning of wood were described by Taylor et al. (1984), Funt and Bryan (1987), and Lindgren (1991), showing that the method can be used for accurate and nondestructive measurements of wood density. In addition, the use of a mobile CT apparatus made it possible to detect the water distribution in stems of living trees (Habermehl et al., 1986(Habermehl et al., , 1990. The main advantage of this mobile method is that changes in the water distribution within a single tree may be monitored over periods of days, months, or years. However, the spatial resolution is low and does not effectively allow measurements of the water relations in individual annual rings.In this study, a new technique is presented that permits the mapping of water concentrations in a whole stem as well as in individual annual rings with a spatial resolution of 0.1225 mm 3 . This has become possible because the same disc is scanned under field-fresh and dry conditions, respectively, so that the density differences can be calculated from image subtraction with data values reflecting the absorption coefficients of water without any cell material. Thus, our method leads to...

The carbonate looping process is an efficient technology for post‐combustion CO2 capture from fossil‐fired power plants. Testing campaigns with continuous CO2 capture were performed in a 1‐MWth carbonate looping pilot plant at the Technische Universität Darmstadt. During two distinguished experimental campaigns the heat for the regeneration of the calcium‐based sorbent in the calciner was provided by propane combustion and combustion of pulverized hard coal, respectively. For several hours of operation, the CO2 concentration in the flue gas was reduced to chemical equilibrium conditions. High overall CO2 capture efficiencies above 90 % were achieved.

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