Flow cytometry has been used to estimate nuclear DNA content of 13 C O~~C C J species (Rubiaceae) investigated. Isolated nuclei from 77 genotypes were stained with propidium iodide (PI: not hase specific). Thirty-nine genotypes were stained with 4',6-diamidino-?-phenylindole (DAPI; AT specific).
CpDNA variation among 52 tree samples belonging to 25 different taxa of Coffea and two species of Psilanthus was assessed by RFLP analysis on both the total chloroplast genome and the atpB-rbcL intergenic region. Twelve variable characters were distinguished allowing the identification of 12 different plastomes. The low sequence divergence observed might suggest that Coffea is a young genus. The results were in contradiction with the present classification into two genera. Additionally, cpDNA inheritance was studied in interspecific hybrids between C. arabica and C. canephora, and in an intraspecific progeny of C. canephora, using PCR-based markers. Both studies showed exclusively maternal inheritance of cpDNA.
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