The family Leguminosae comprises approximately 20,000 species that mostly form symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing bacteria (NFB). This study is aimed at investigating and confirming the dependence on nodulation and biological nitrogen fixation in the specie Piptadenia gonoacantha (Mart.) Macbr., which belongs to the Piptadenia group. Two consecutive experiments were performed in a greenhouse. The experiments were fully randomized with six replicates and a factorial scheme. For the treatments, the two AMF species and three NFB strains were combined to nodulate P. gonoacantha in addition to the control treatments. The results indicate this species’ capacity for nodulation without the AMF; however, the AMF + NFB combinations yielded a considerable gain in P. gonoacantha shoot weight compared with the treatments that only included inoculating with bacteria or AMF. The results also confirm that the treatment effects among the AMF + NFB combinations produced different shoot dry weight/root dry weight ratios. We conclude that AMF is not necessary for nodulation and that this dependence improves species development because plant growth increases upon co-inoculation.
Several tropical woody species are highly responsive to arbuscular mycorrhizal fungi (AMF) and depend on their symbiosis for nutrition and successful establishment in the field. The objective of this study was to evaluate the response of Schinus terebinthifolius, a native species of the Brazilian Atlantic Forest and Caatinga, to inoculation with three AMF species (Dentiscutata heterogama, Gigaspora margarita, and Rhizophagus clarus), either individually or mixed, at different levels of phosphorus fertilization (0, 71, 213, and 650 mg kg−1 of P). We conducted the experiment in 1 kg pots, following a completely randomized design with six replicates per treatment, to evaluate morphological and nutritional traits after 116 days, including stem diameter, plant height, plant biomass, and shoot P content. Our results showed that S. terebinthifolius was highly dependent on mycorrhizae and presented different responses depending on the AMF species. The greatest total biomass accumulation occurred when a mixture of the three AMF species was used, which indicated synergism between the fungi. The highest overall positive response to inoculation were observed at an intermediate P level (213 mg kg−1), and although shoot biomass was reduced at the intermediate P doses, an increase in root biomass compensated for this. These results indicate complementarity as opposed to functional redundancy of the AMF species and highlight the importance of using a mixed inoculum in seedling production of S. terebinthifolius for revegetation programs.
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