Objectives. This study was conducted to determine which admissions criteria are valuable in selecting pharmacy students by determining which criteria are significant predictors of success or failure. Methods. A statistical analysis was conducted on the retrospective data of 309 students. Academic probation was used as an indication of academic failure. Academic success was measured by first-professional year grade point average (GPA) in pharmacy courses. Results. Predictors of failure included ACT composite score, average grade in organic chemistry courses, and gender. Predictors of success included ACT score, average grade in organic chemistry courses, grades in math and science prepharmacy courses, and prior attainment of a bachelor's degree. Conclusion. Academic predictors of success and failure shared common variables, but there were predictors of success that were not predictors of failure. It may be useful for selection committees to consider both sets of predictors as part of the screening processes.

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Perfusion of cat paws with compound 48/80 released two slow reacting substances (SRSs) which were isolated and characterized as 5-hydroxy-S-cysteinylglycyl-7,9,-11,14-icosatetraenoic acid (SRS I) and -hydroxy-6S-cysteinyl-7,9,11,14-icosatetraenoic acid (SRS II) on the basis of chemical degradations, amino acid analyses, spectroscopic and enzymic experiments, and comparison with synthetic samples. The smooth muscle-contractile activities of synthetic 5-hydroxy-6 y-glutamylcysteinylglycyl-7,9,11,14-icosatetraenoic acid, synthetic5-hydroxy--S-cysteinyl-7,9,11,14-icosatetraenoic acid, and SRS II were not inactivated by arylsulfatase. On the other hand, the spasmogenic activities produced by synthetic 5-hydroxy-6S-cysteinylglycyl-7,9,11,14-icosatetraenoic acid and SRS I were destroyed at the same rate by the arylsulfatase. This mode of inactivation was attributed to an aminopeptidase activity in the arylsulfatase preparation because 5-ydroxyl-6-Scysteinyl-7,9,11,14-icosatetraenoic acid was isolated and identified as the reaction end product. Because the properties of SRS from cat paws closely resemble those of SRS generated by immunological stimulation of human tissues (SRS-A) and because all known SRS-A are inactivated by arylsulfatases, we contend that 5-hydroxy-6S-cysteinylglycyl-7,9,11,14-icosatetraenoic acid (SRS I) corresponds to SRS-A.In 1938, Feldberg and Kellaway (1) first described a substance that caused the guinea pig ileum to contract more slowly and with more sustained action than did histamine; they named the compound "slow reacting substance (SRS)." Two years later, Kellaway and Trethewie (2) reported that slow reacting substance of anaphylaxis (SRS-A) was released from sensitized tissue by a specific antigen. Subsequently, it was shown (3) that SRS-A was one of several pharmacologic mediators that may be important in human asthma. SRS has since been found to be released from many types of tissues and cell suspensions including guinea pig, rabbit, monkey, bovine, and human lungs (4-6), cat paws (7,8), isolated rat mast cells (9), human leukocytes (10) and nasal polyps (11), human and rat leukemic basophils (12,13), and rat peritoneal fluid (14, 15).Here we present our studies on the purification and identification of two SRSs from cat paws after perfusion with Compound 48/80 (16) (,uC18) preparative column (0.94 X 50 cm) of Partisil-10 M9 ODS (Whatman) was used for SRS purification. The radial compression separation system consisted of a Waters radial compression module (RCM-100) with a radial-Pak C18 cartridge (0.8 X 10 cm).SRS Assay. SRS bioactivity was assayed on guinea pig ileum segments by a modification of the procedure reported by Chakravarty (17). Histamine was assayed in the presence of atropine (1 MM) and SRS was assayed in the presence of atropine (1 ,uM) and mepyramine (1 ,M). One unit of SRS was defined as that amount of SRS which caused a contraction with a peak height equal to that induced by 5 ng of histamine base. After the log-dose-response curve for histamine was determin...

A liquid chromatographic (LC) method is described for the assay of tylosin (tylosin A, tylosin B, and tylosin-urea adduct [TUA]) in animal feeds at approximately 4-200 g/ton. Samples are extracted with an equal mixture of methanol and 0.1 M pH 8 phosphate buffer. An acidic alumina column cleanup step is used prior to reversed-phase LC. Determination is accomplished with UV detection at 280 nm. A15 cm C8 column is used for sample concentration. Elution is performed at 1.5 mL/min with a gradient containing an increasing methanol concentration with 0.5% acetic acid and tetramethylammonium chloride (5 g/L) as the aqueous portion. Baseline separation of tylosin B, TUA, and tylosin A is achieved with retention times of approximately 7.4, 9.3, and 10.7 min, respectively. Conversion factors of 1.0 and 1.2 are used to convert peak areas of tylosin B and TUA, respectively, to tylosin A equivalent as an alternative to standards being used for these components of some feeds. The precision of the procedure ranged from a coefficient of variation (CV) of 1.6% for tylosin A at 94 g/ton to a CV of 11.3% for TUA at 10 g/ton. The average percent recovery of spiked feed extracts was100.4-102.2% for the 3 tylosin components. A correlation with the microbiological assay is presented for 12 feeds, 1 premix, and 1 injectable tylosin product.

A liquid chromatographic (LC) method is described to assay chlortetracycline (CTC) at gram-per-ton and gram-per-pound levels. Feeds and premixes are extracted with acid-acetone following AOAC Method 957.23B(h). Extracts are filtered and quantitated by LC using a Ciβ column and fluorescence detection at 390 nm excitation and 512 nm emission. The mobile phase (1.5 mumin) uses a methanol gradient with an initial hold time of 5 min at 35% methanol followed by an increase to 60% methanol over 8 min. The aqueous component contains CaCI2, disodium EDTA, and sodium acetate buffered to pH 6.5. The method provides significant improvement in selectivity and limit of quantitation compared to LC with UV detection. Recovery of spiked samples averaged 98.1% and assay of samples correlated closely with the results obtained with AOAC microbiological Method 967.39. CTC and epi-CTC eluted at approximately 12.5 and 4.0 min, respectively. No interference was observed from several drugs and other antibiotics often used in combination with CTC in feeds.