More than 70% of all knee injuries in soccer occur in non-contact situations. It is known that increased lower limb dynamic knee valgus is associated with such situations. Little has been found out about differences in knee kinematics of the dominant (kicking) and non-dominant (supporting) leg during a single leg landing. A total of 114 male adolescent soccer players (age 14.6 ± 1.1 years) from elite (N = 66) and amateur soccer clubs (N = 48) performed a single leg drop landing down from a box. For each leg, the two-dimensional dynamic knee valgus angle (DKVA) was calculated. Paired t-tests were used to statistically determine significant differences between dominant and non-dominant leg DKVA, and t-tests were calculated between the two performance groups. Statistically significant differences (p < 0.05) were identified for the DKVA between the dominant and non-dominant leg for both amateur and elite players, showing a greater DKVA for the dominant leg. Group differences for the DKVA between amateur and elite players were not found, neither for the dominant, nor for the non-dominant leg. It can be concluded that the non-dominant leg showed more stable dynamics than the dominant leg during unilateral landing regardless of the player’s performance level. This could be due to adaptions to sport-specific requirements. Therefore, it is recommended that programs to prevent knee injuries among soccer players consider the dynamics of each leg individually.