The storage and survival of 97 species (276 strains) of bacteria at – 70° to – 80°C was investigated. Each strain was subcultured at regular intervals over a period of 6–40 months in order to check its viability. All strains were viable after the latest subculture except strains of Neisseria gonorrhoeae which proved non‐viable when tested after 12 months. The method proved most useful for storing stock cultures which required frequent and regular accessing.
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