Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.

Marine microbes along with microeukaryotes are key regulators of oceanic biogeochemical pathways. Here we present a high-resolution (every 0.5° of latitude) dataset describing microbial pro- and eukaryotic richness in the surface and just below the thermocline along a 7,000-km transect from 66°S at the Antarctic ice edge to the equator in the South Pacific Ocean. The transect, conducted in austral winter, covered key oceanographic features including crossing of the polar front (PF), the subtropical front (STF), and the equatorial upwelling region. Our data indicate that temperature does not determine patterns of marine microbial richness, complementing the global model data from Ladau et al. [Ladau J, et al. (2013) ISME J 7:1669-1677]. Rather, NH, nanophytoplankton, and primary productivity were the main drivers for archaeal and bacterial richness. Eukaryote richness was highest in the least-productive ocean region, the tropical oligotrophic province. We also observed a unique diversity pattern in the South Pacific Ocean: a regional increase in archaeal and bacterial diversity between 10°S and the equator. Rapoport's rule describes the tendency for the latitudinal ranges of species to increase with latitude. Our data showed that the mean latitudinal ranges of archaea and bacteria decreased with latitude. We show that permanent oceanographic features, such as the STF and the equatorial upwelling, can have a significant influence on both alpha-diversity and beta-diversity of pro- and eukaryotes.

[1] Scientists vigorously debate the degree to which rock varnish is formed through the actions of microorganisms. To investigate this enigma, we utilized a three-pronged approach that combined (1) culture-independent molecular methods to characterize bacterial communities associated with varnish that coats the rhyolitic volcanic rocks of Black Canyon, New Mexico, and rocks with no visible varnish; (2) culturing of varnish in media supplemented with reduced forms of manganese and/or iron and no or low amounts of carbon to isolate bacteria capable of precipitating iron and/or manganese oxides; and (3) scanning electron microscopy (SEM) of varnish and nearby rock that lacks macroscopically visible varnish. Our culture-independent studies revealed significant differences between varnish and nonvarnish communities. Chloroflexi and Ktedobacteria dominated one varnish site, while the other varnish site was dominated by Cyanobacteria. The nonvarnish sites were dominated by Actinobacteria and, to a lesser extent, Cyanobacteria and were the only samples to contain Deinococcus-Thermus sequences. Approximately 65% of varnish cultures produced visible manganese precipitates. Most culture isolates were not closely related to known manganese oxidizers, with the exception of Bacillus spp. SEM revealed microbial morphologies and two types of varnish morphologies: (1) relatively smooth layers and (2) patches of botryoidal pinnacles, which were often associated with increased manganese concentrations. "Bare" rock showed evidence of incipient varnish. These results have important implications for the detection of life on extraterrestrial planets such as Mars, where putative varnish coatings have been observed, and represent some of the first culture-independent characterizations of varnish communities.

Sustained observations of microbial dynamics are rare, especially in southern hemisphere waters. The Australian Marine Microbial Biodiversity Initiative (AMMBI) provides methodologically standardized, continental scale, temporal phylogenetic amplicon sequencing data describing Bacteria, Archaea and microbial Eukarya assemblages. Sequence data is linked to extensive physical, biological and chemical oceanographic contextual information. Samples are collected monthly to seasonally from multiple depths at seven sites: Darwin Harbour (Northern Territory), Yongala (Queensland), North Stradbroke Island (Queensland), Port Hacking (New South Wales), Maria Island (Tasmania), Kangaroo Island (South Australia), Rottnest Island (Western Australia). These sites span ~30° of latitude and ~38° longitude, range from tropical to cold temperate zones, and are influenced by both local and globally significant oceanographic and climatic features. All sequence datasets are provided in both raw and processed fashion. Currently 952 samples are publically available for bacteria and archaea which include 88,951,761 bacterial (72,435 unique) and 70,463,079 archaeal (24,205 unique) 16 S rRNA v1-3 gene sequences, and 388 samples are available for eukaryotes which include 39,801,050 (78,463 unique) 18 S rRNA v4 gene sequences.