From the analysis of 230 scats, the diet of the maned wolves, Chrysocyon brachyurus (Illiger, 1815), was determined in a private natural reserve in southeastern Brazil in which ecotourism activities are developed and the animals are deliberately fed bovine meat. A total of 569 occurrences of food items were recorded, of which 56.8% were of animal origin and 29.1% of vegetal origin. Rodents, insects and birds added up to 35.8% of the occurrences, yet accounted for 68.5% of the total number of preys (277). Insects, however, had practically no importance (0.1%) in the total estimated biomass consumed. Even though the fruit Solanum lycocarpum St.-Hil. is a common food item in the diet of the maned wolf, its occurrence in the diet at the Serra do Caraça Reserve was insignificant, accounting for only 4.8% of the total number of food item occurrences and 3.4% of the total estimated biomass consumption. Food items of anthropic origin and inorganic items (e.g., plastic) represented 14.1% of all occurrences, which shows that the animals are used to the presence of humans. Seasonal variations in consumption were found for S. lycocarpum (c² = 10,09; p < 0,001), for other fruits (c² = 19,73; p < 0,001), and for reptiles (c² = 15,56; p < 0,001), all of which were more frequently eaten during the dry months. There was a significant correlation between the availability of small mammals and their consumption by the maned wolves (r s = 0.59; p = 0.041), yet the same was not observed for the fruits of S. lycocarpum (r s = 0,101; p = 0,754). Our findings stress the need for a better understanding of the effects of additional foods on the natural feeding habits of the maned wolf
