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Assessing the viability of human pancreas grafts using 31 P MR spectroscopy L. Carlbom, J. Weis, A. Biglarnia, O. Korsgren, H. Ahlström; Uppsala/SE (lina.carlbom@radiol.uu.se) Purpose: In order to select pancreas suitable for transplantation an objective non-invasive assessment of graft viability is desirable. 31P magnetic resonance spectroscopy ( 31 P-MRS) has previously been applied for pancreas graft evaluation in animal models, with promising results. The aim of this pilot study was to investigate the feasibility of using 31 P-MRS for assessment of human pancreas graft viability prior to transplantation. Methods and Materials: Pancreata from five human donors were included. Immediately after removal from the donor each pancreas was perfused with histidine-tryptophan-ketoglutarate (HTK) solution and stored in hypothermic condition (4 o C). 31P-MRS was performed on a 1.5 T clinical MR scanner, using an ISIS sequence. Typical voxel size was ~5×5×14 cm 3 . The first spectrum was acquired 6-10 hours after HTK perfusion start. During the following 17-25 hours subsequent spectra were obtained by repeated examinations, while preserving the hypothermic environment. Thereafter the pancreas was exposed to room temperature for 24 hours and the last spectrum was measured. This spectrum served as a reference for non-viable tissue. The following metabolites were fitted: phosphomonoesters (PME), inorganic phosphate (Pi), phosphodiesters (PDE), phosphocreatine (PCr), and adenosine triphosphate (ATP). Results: -ATP and -ATP lines decreased to the noise level within 2-4 hours after the start of 31 P-MRS. PME, PDE and -ATP levels gradually decreased and Pi increased. Non-viable pancreas tissue revealed dominant Pi and small PME and PDE intensities. Conclusion: ( -ATP+ -ATP)/Pi, PME/Pi and PDE/Pi spectral intensity ratios obtained by 31 P-MRS are promising quantitative parameters for objective noninvasive assessment of the viability of human pancreas grafts. B-0002 10:39Utility of DWI sequences in autoimmune pancreatitis before and after steroid therapy B. Pedrinolla, A. Cybulski, R. Negrelli, S. Mehrabi, R. Manfredi, R. PozziMucelli; Verona/ IT (beatrice.pedrinolla@gmail.com) Purpose: To evaluate the utility of Diffusion Weighted (DWI) sequences in Autoimmune Pancreatitis (AIP) and to identify typical values of apparent diffusion coefficients (ADC) before and after steroid therapy. Methods and Materials: Between February 2010 and April 2013 we evaluated retrospectively 35 Magnetic Resonance examination with Diffusion Weighted Imaging (DWI) Sequences in 25 patients with diagnosis of AIP (19 men, 6 women, mean age 45.7 years). Ten out of 25 patients performed MRI before and after steroid therapy, 3/25 only before and 12/25 only after therapy. Two Radiologists separately analysed each exam, evaluating the signal ntensity of the affected parenchyma in T1-weighted and DWI sequences and measuring ADC values, through circular region of interest. Results: In the acute phase AIP appeared hypointense in T1 sequences in 13/13 (100%)...

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