Toxoplasma gondii causes toxoplasmosis, a worldwide disease. Experimentation with pigs is necessary for the development of new therapeutic approaches to human diseases. BR-1 mini pigs were intramuscularly infected with T. gondii with tachyzoites (RH strain) or orally infected with cysts (ME-49 strain). Haematology and serum biochemistry were analysed and buffy coat cells were inoculated in mice to determine tachyzoite circulation. No alterations were observed in erythrocyte and platelet values; however, band neutrophils increased seven days after infection with ME-49. Serology of the mice inoculated with pig blood leucocytes revealed circulating ME-49 or RH strain tachyzoites in the pigs' peripheral blood at two and seven or nine days post-infection. The tachyzoites were also directly observed in blood smears from the infected pigs outside and inside leucocytes for longer periods. Alanine-aminotransferase was high at days 21 and 32 in the RH infected pigs. After 90 days, the pigs were euthanised and their tissue samples were processed and inoculated into mice. The mice serology revealed the presence of parasites in the hearts, ileums and mesenteric lymph nodes of the pigs. Additionally, cysts in the mice were only observed after pig heart tissue inoculation. The infected pigs presented similar human outcomes with relatively low pathogenicity and the BR-1 mini pig model infected with ME-49 is suitable to monitor experimental toxoplasmosis.
O presente estudo objetiva relatar o tratamento de uma úlcera corneana indolente em equino, através do emprego associado de debridamento do tecido epitelial necrótico com broca de diamante e medicação tópica. Os sinais clínicos foram confirmados pelo exame com fluoresceína em strips. No dia 0, o cavalo foi anestesiado topicamente por cloridrato de proximetacaína 0,5% (Anestalcon®) e foi realizado o debridamento com uma broca de diamante manual (Algerbrush II®) de cabeça média. O tratamento medicamentoso envolveu a administração de soro autólogo refrigerado e colírios à base de moxifloxacino (Vigamox®) e de ácido hialurônico (Hyabak®), ambos de 4 em 4 horas, por 14 dias, com revisões nos dias 7 e 14, mantendo-se o animal confinado para evitar a ocorrência de possíveis traumas e complicações. Nas revisões, observou-se o progresso efetivo do tratamento, com avanço favorável da reepitelização da córnea como esperado no dia 7. No dia 14 a córnea já se encontrava completamente reepitelizada, possibilitando que se reputasse o tratamento como totalmente bem sucedido. Concluiu-se que a abordagem de tratamento empregada se demonstrou eficaz para a completa cicatrização da córnea e o fez de forma rápida, um objetivo a ser buscado particularmente quando o caso em questão envolve animais utilizados no desempenho de atividades laborais.
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