No abstract

Temperature‐response information for use in evaluating thermal discharges is often over 30 years old and in the nonpeer‐reviewed literature, especially for Round Whitefish Prosopium cylindraceum and Lake Whitefish Coregonus clupeaformis exposed to nonlethal, elevated, and variable temperatures. Egg incubation experiments on Round Whitefish collected in Lake Ontario and Lake Whitefish collected in Lake Huron were carried out from December 13, 2011, to April 7, 2012. Experimental treatments included ambient baseline control conditions as well as fixed and fluctuating (variable) temperature increases of 1, 2, 3, and 5°C above ambient baseline conditions. For both species, the window for hatching for all experimental temperature treatments was variable (range, 10–38 d for Round Whitefish and 11–44 d for Lake Whitefish), and the hatching windows tended to be greater as temperatures increased. Our results indicated that both fixed and variable incremental increases in temperature above ambient baseline conditions have a statistically significant effect on 50% hatch, and hatch occurs earlier with higher incremental temperature increases. The ecological significance of advanced hatch, such as indirect mortality and food source availability, was evaluated. Received February 19, 2013; accepted July 8, 2013

-The insecticidal properties of biotin-binding proteins (BBPs) have recently been exploited in transgenic plants. As BBPs have a broad spectrum of insect toxicity, their potential impacts on non-target insects such as honey bees need to be assessed. In this study, the effects of feeding a purified BBP, avidin, to honey bee larvae and adults were determined. A realistic larval dosing regime was developed by estimating the pollen content of brood food in the field and adding avidin to artificial diet at rates that simulated the presence of avidin-expressing transgenic pollen in brood food. Larval survival and development were unaffected by avidin in assays which simulated larvae receiving pollen expressing 0, 4 or 40 µM avidin at concentrations of 164 µg pollen per mg food for the first 2 days and 880 µg pollen per mg food thereafter. Food consumption and survival of adult bees were also unaffected by avidin added to pollen-candy at levels corresponding to pollen expression of 0, 6.7 or 20 µM avidin.Apis mellifera / biotin-binding protein / avidin / transgenic plant

Two glasshouse trials in a capsicum crop near Warkworth New Zealand in January 2011 and two field trials in a nectarine orchard near Lleida Spain in July 2013 were undertaken to determine if trap capture of Frankliniella occidentalis (western flower thrips WFT) and Thrips tabaci (onion thrips OT) could be increased by alternative volatile compounds beyond the known thrips attractant methyl isonicotinate (MI) On blue sticky board traps in the crop in New Zealand none of six alternative compounds tested caught more thrips (mostly WFT OT and two other species) than the Control traps In contrast to MI catches with the alternative lures were mostly significantly lower For both trials in the orchard in Spain using white water traps the highest numbers of WFT and OT were caught higher than most of the nine alternative odour compounds tested

We have identified lipase-like genes from an Epiphyas postvittana larval midgut EST library. Of the 10 pancreatic lipase family genes, six appear to encode active lipases and four encode inactive lipases, based on the presence/absence of essential catalytic residues. The four gastric lipase family genes appear to encode active proteins. Phylogenetic analysis of 54 lepidopteran pancreatic lipase proteins resolved the clade into five groups of midgut origin and a sixth of non-midgut lipases. The inactive proteins formed two separate groups with highly conserved mutations. The lepidopteran midgut lipases formed a ninth subfamily of pancreatic lipases. Eighteen insect and human gastric lipases were analysed phylogenetically with only very weak support for any groupings. Gene expression was measured in the larval midgut following feeding on five artificial diets and on apple leaves. The artificial diets contained different levels of triacylglycerol, linoleic acid and cholesterol. Significant changes in gene expression (more than 100-fold for active pancreatic lipases) were observed. All the inactive lipases were also highly expressed. The gastric lipase genes were expressed at lower levels and suppressed in larvae feeding on leaves. Together, protein motif analysis and the gene expression data suggest that, in phytophagous lepidopteran larvae, the pancreatic lipases may function in vivo as galactolipases and phospholipases whereas the gastric lipases may function as triacylglycerol hydrolases.