Rumex hastatulus is the North American endemic dioecious plant with heteromorphic sex chromosomes. It is differentiated into two chromosomal races: Texas (T) race characterised by a simple XX/XY sex chromosome system and North Carolina (NC) race with a polymorphic XX/XY1Y2 sex chromosome system. The gross karyotype morphology in NC race resembles the derived type, but chromosomal changes that occurred during its evolution are poorly understood. Our C-banding/DAPI and fluorescence in situ hybridization (FISH) experiments demonstrated that Y chromosomes of both races are enriched in DAPI-positive sequences and that the emergence of polymorphic sex chromosome system was accompanied by the break of ancestral Y chromosome and switch in the localization of 5S rDNA, from autosomes to sex chromosomes (X and Y2). Two contrasting domains were detected within North Carolina Y chromosomes: the older, highly heterochromatinised, inherited from the original Y chromosome and the younger, euchromatic, representing translocated autosomal material. The flow-cytometric DNA estimation showed ∼3.5 % genome downsizing in the North Carolina race. Our results are in contradiction to earlier reports on the lack of heterochromatin within Y chromosomes of this species and enable unambiguous identification of autosomes involved in the autosome-heterosome translocation, providing useful chromosome landmarks for further studies on the karyotype and sex chromosome differentiation in this species.Electronic supplementary materialThe online version of this article (doi:10.1007/s10577-014-9446-4) contains supplementary material, which is available to authorized users.

The purpose of this study is to investigate anti-EU and anti-LGBT attitudes in Poland on the basis of quantitative evidence (statistical data) and qualitative evidence (discourse analysis of statements expressed on the Internet). As Euroscepticism seems to frequently appear in conjunction with prejudice against LGBT (lesbian, gay, bisexual and transsexual or transgender) persons, the task of this article is to find out whether they may have a common foundation and what it may be.A possible answer, as the article argues, is that both attitudes could be considered symptoms of a deeper, more wide-ranging and fundamental problem-a fear, tension, or anxiety caused by social change, especially the fragmentation of dominant collective (national) identity. The case for such an interpretation of the situation is first made on the basis of existing academic literature and statistical data provided by Eurobarometer and the Polish Public Opinion Research Centre (Centrum Badania Opinii Społecznej, CBOS). However, as the text further argues, such quantitative methods have their limitations and should be supported and illustrated with qualitative studies. The article thus proposes an alternative discourse-oriented approach, namely critical discourse analysis (CDA). This approach is used to conduct an introductory, presentational analysis of some examples of anti-EU and anti-LGBT discourse found on the Internet. This analysis shows that sexual minorities represent values so strange and foreign to Polish conservatives that they can only be conceptualized as something imposed by the power which is both new and distant-by Brussels. And the other way round, the European Union's liberalism and espousal of human rights, including women's and LGBT rights, makes it impossible for the conservative parts of the Polish society to accept a "European identity". This means that Euroscepticism and LGBT prejudice are not just occasionally, coincidentally connected expressions of an underlying resistance to change, but that a closer relationship exists between them. Namely, the conservative reluctance or hostility towards both the EU and LGBT is caused by their incompatibility with the patriotic and religious national identity construction.

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