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All the parameters measured showed similar improvement such as PPD reduction, CAL gain, IBD reduction and defect resolution with OFD + DFDBA + AM and OFD + DFDBA, no statistical significant difference was observed between the two groups .

A 45-year-old male patient reported to the Department of Periodontology, Krishnadevaraya College of Dental Sciences and Hospital, Bangalore for management of dull and nagging pain since 4 days in 45 and 46. Intraoral examination revealed generalized periodontal pockets (4-7 mm), Grade II furcation involvement in 46 and localized periodontal abscess in respect to 44 and 45. Radiographically, 44 and 45 demonstrated moderate angular bone loss with a deeper component distally. The case was diagnosed as moderate chronic periodontitis with chronic localized periodontal abscess at 44 and 45. A comprehensive treatment plan including non-surgical and presumptive surgical therapy including use of LLLT and bone graft was presented before the patient and signed informed consent was obtained.On day 1, intra-sulcular emergency abscess drainage was carried out at 44 and 45. Non-surgical periodontal therapy consisting of full mouth scaling and root planing was completed within next three weeks, with follow-up up of three months. The clinical parameters were recorded [Table/ Fig-1,2]. Observing optimal plaque control, good patient compliance and deep residual pockets, decision for surgical treatment was made and informed to the patient.

Introduction: Cryosurgery is a branch of therapeutics that makes use of local freezing for the controlled destruction or removal of living tissues. Cryosurgery has been tried with satisfactory results for gingival depigmentation. However, documented evidence in the literature is extremely limited. The present case report describes the successful management of excessive gingival pigmentation by cryosurgery. The patient underwent follow‐up for 30 months.Case Presentation: A 21‐year‐old South Indian dark‐skinned female presented with dark brown to black gingival melanin pigmentation. The darkly pigmented gingiva had been present since birth. Cryosurgery using nitrous oxide and gas expansion cryoprobe cooled to −70°C was used for the depigmentation. The depigmentation was performed from maxillary right cuspid to maxillary left cuspid in a single appointment. The patient reported no adverse effects and no repigmentation of the treated areas for a period of 30 months.Conclusions: Cryosurgery is an easy procedure, with lack of bleeding and scar formation. The esthetic outcome may be maintained for 30 months as shown in the present case. Cryosurgery can be considered a desirable treatment option for gingival depigmentation.

The aim of the present study was to evaluate the influence of recombinant platelet derived growth factor-BB (rhPDGF-BB) on the cellular proliferation, morphology and adherence of human periodontal ligament fibroblasts (PLFs) to the root surfaces of periodontally diseased and healthy human teeth roots at two different time periods. Primary cell cultures of PLFs were obtained from clinically healthy premolar or mandibular third molar teeth. 11 scaffolds were prepared from healthy teeth for group-1 (Healthy Substrates), 33 scaffolds were prepared from periodontally diseased teeth, which were further divided in Group-2 - Periodontally diseased substrates, Group-3 - Scaled and Root planed (SRP) substrates, Group-4 - SRP + rhPDGF-BB (50 ng/ml). Groups were further subdivided into two groups (n = 5 scaffolds per subgroup) and PLFs were incubated on the scaffolds for three and seven days, topographical assessment was done on the remaining substrate. Cell morphology and counting was assessed under a scanning electron microscope at 350× on day three and seven and statistically compared with the Mann-Whitney U test and the Kruskal-Wallis test. On day three, Group 1 showed least number of cells attached, whereas maximum number of cells were attached on Group 3 (SRP only) substrates. For day 7, Group 1 and Group 4 showed increase in the number of cells from day 3 to 7, while number of cells attached/substrate reduced drastically for Group 2 and 3 substrates. Group 3 and 4 showed better adhesion and proliferation of PLFs as compared to Group 1 and 2. Group-1 and Group-4 showed predominantly spindle cells with flat appearance, Group-3 showed stellate cells and Group-2 showed predominantly distorted spindle shaped cells. The results of this in-vitro study indicates that rhPDGF-BB plays a significant role as an adjunct to periodontal therapy in influencing maturity, attachment and proliferation of PLFs.

Background and ObjectivesThe purpose of this first of its kind study was to analyse the growth, development and attachment of cultured human umbilical cord stem cells alone or supplemented with basic Fibroblast Growth Factor (bFGF) on both healthy and periodontally diseased tooth surfaces in vitro.MethodsFour groups of 12 root surface scaffolds each were classified as Group I- healthy root surfaces; Group II- periodontally diseased; Group III- Healthy with bFGF and Group IV- periodontally diseased root with bFGF. bFGF was applied in the concentration of 8 ng/ml on to the surface followed by incubation of cultured human umbilical cord stem cells (hUCMSCs) on the scaffolds. Scanning electron microscopy observations were made on 14th and 21st days to assess the proliferation and morphology of cells attached on the tooth surface.ResultsCultured hUCMSCs demonstrated adhesion to tooth root scaffold. All the groups showed a significant increase in the number of cell attachment from 14th day to 21st day. The groups with bFGF showed a significant increase in attachment of cells when compared to the groups without bFGF. The cells showed an increase in number of flat cells from 14th day to 21st day in all the groups indicating an increased maturity of cells. Periodontally diseased groups had less maturity of cells than healthy groups. The groups supplemented with bFGF, had more mature cells than the groups without bFGF.ConclusionshUCMSCs have the propensity to differentiate into cells that have the capacity to bind to root surfaces. hUCMSCs incubated with bFGF showed better proliferation and attachment to tooth root surfaces. The role of hUCMSCs can be further explored for periodontal regeneration.

Clarithromycin can attain higher levels in gingiva than serum of patients with periodontitis. This distribution profile of clarithromycin can thus be advantageous in the management of periodontal lesions.