Vilanova JM, Figueras J, Roselló J, Gómez G, Gelpí E, Jiménez R. Arachidonic acid metabolites in CSF in hypoxic-ischaemic encephalopathy of newborn infants. Acta Paediatr 1998; 87: 588-92. Stockholm. ISSN 0803-5253The aim of this study was to evaluate the cerebral synthesis of eicosanoids in the asphyctic newborn and to investigate the relation between the prostanoid profiles in cerebrospinal fluid (CSF) and the appearance and severity of hypoxic-ischaemic encephalopathy (HIE). Levels of 6-keto-PGF 1-a , TXB 2 , PGE 2 and PGF 2-a in CSF were measured in 40 full term newborns during the first day of life. Thirty of these newborns had birth asphyxia and were divided into three groups: 10 without HIE, 12 with mild HIE and 8 with moderate-severe HIE. They were compared to a control group of 10 non-hypoxic newborns. Determinations of the metabolites in CSF were performed by RIA and expressed as pg/ml (mean Ϯ SD). The CSF TXB 2 (thromboxane A 2 metabolite) in asphyxiated newborns was always higher than in the control group (28.12 Ϯ 10.6), and related to the severity of HIE ( p ¼ 0:005): without HIE (50.84 Ϯ 16.4; p ¼ 0:02), mild HIE (80.65 Ϯ 12.64; p Ͻ 0:01) and moderate-severe HIE (178.14 Ϯ 20.5; p Ͻ 0:01). The CSF 6-keto-PGF 1-a (prostacyclin metabolite) in asphyxiated newborns was always higher than in the control group (80.55 Ϯ 12.56), but indirectly related to the severity of HIE: without HIE (240.95 Ϯ 28.12; p Ͻ 0:01), mild HIE (183.65 Ϯ 30.1; p Ͻ 0:01) and moderate-severe HIE (140.55 Ϯ 25.12; p Ͻ 0:01). In the moderatesevere HIE group, the increase in TXB 2 was higher than the rise in 6-keto-PGF 1-a . ٖ Eicosanoids, hypoxicischaemic encephalopathy, newborn, perinatal asphyxia, prostaglandins, prostanoids J Figueras-Aloy,
Fasted 1-day-old rat liver has high heparin-releasable (endothelial) lipoprotein lipase (LPL) activity, and its hepatocytes synthesize LPL protein. To test the physiological role of this LPL, we perfused the isolated organ with a 0.8 mM triacylglycerol (TAG) (Intralipid + glycerol tri[3H]oleate) 6.3% serum medium. Samples of the recirculated perfusate were taken at different times to determine 3H in TAG, free fatty acid (FFA), and water-soluble (WS) fractions. In the medium [3H]TAG disappeared and [3H]FFA and [3H]WS fractions appeared linearly with time. This TAG hydrolysis was 1) absent when medium was recirculated without liver, 2) not affected by chloroquine addition, 3) inhibited by anti-LPL immunoglobulins, 4) absent when serum was omitted from the medium, and 5) restituted when apolipoprotein CII was added to the medium without serum. Therefore, lysosomal lipase is not involved in this TAG hydrolysis, the features of which are characteristic of LPL, not of the so-called "hepatic endothelial lipase." Thus LPL activity enables the neonatal rat liver to hydrolyze and take up circulating TAG, i.e., has the same function as extrahepatic LPL.
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