The incidence of inflammatory bowel disease is increasing worldwide and the underlying molecular mechanisms are far from being fully elucidated. Herein, we evaluated the role of N-glycosylation dysregulation in T cells as a key mechanism in the ulcerative colitis (UC) pathogenesis. The evaluation of the branched N-glycosylation levels and profile of intestinal T cell receptor (TCR) were assessed in colonic biopsies from UC patients and healthy controls. Expression alterations of the glycosyltransferase gene MGAT5 were also evaluated. We demonstrated that UC patients exhibit a dysregulation of TCR branched N-glycosylation on lamina propria T lymphocytes. Patients with severe UC showed the most pronounced defect on N-glycan branching in T cells. Moreover, UC patients showed a significant reduction of MGAT5 gene transcription in T lymphocytes. In this study, we disclose for the first time that a deficiency in branched N-glycosylation on TCR due to a reduced MGAT5 gene expression is a new molecular mechanism underlying UC pathogenesis, being a potential novel biomarker with promising clinical and therapeutic applications.
Analisar o conhecimento dos preceptores na utilização de metodologias ativas durante estágio supervisionado. Estudo descritivo com abordagem quantitativa. A pesquisa foi realizada através de questionário contendo questões sociodemográficas e objetivas sobre o tema. Participaram preceptores do curso de graduação em enfermagem de uma universidade particular. A amostra do estudo foi composta por 10 preceptores, todos do sexo feminino. 9 preceptores possuem especialização em áreas distintas e 1 possui título de mestre. Apenas 5 preceptores foram capacitados para atuar na preceptoria. Todos utilizam metodologias ativas no campo de estágio e apenas 6 receberam treinamento. Evidenciou-se que o enfermeiro preceptor tem um papel importante na formação de futuros profissionais, encarando o discente como protagonista do aprendizado. O uso das metodologias ativas nos estágios é relevante, pois envolve os desafios existentes na profissão, desde os estruturais até os pedagógicos.Descritores: Preceptor, Estágio Supervisionado, Metodologias Ativas. Nursing preceptorship: use of active methodologies during supervised internshipAbstract: To analyze the knowledge of preceptors in the use of active methodologies during supervised internship. Descriptive study with quantitative approach. The research was conducted through a questionnaire containing sociodemographic and objective questions on the subject. The participants of the undergraduate nursing course at a private university participated. The study sample consisted of 10 preceptors, all female. 9 preceptors have specialization in different areas and 1 has a master's degree. Only 5 preceptors were trained to act in preceptorship. It all uses active methodologies in the internship field and only 6 received training. It was evidenced that the preceptor nurse has an important role in the training of future professionals, facing the student as the protagonist of their learning. The use of active methodologies in internships is relevant, as it involves the challenges existing in the profession, from structural to pedagogical.Descriptors: Preceptor, Supervised Internship, Active Methodologies. Preceptoría de enfermería: uso de metodologías activas durante la pasantía supervisadaResumen: Analizar el conocimiento de los preceptores en la utilizaión de metodologías activas durante las pasantías supervisadas. Estudio descriptivo con enfoque cuantitativo. La investigación se llevó a cabo a través de un cuestionario que contiene preguntas sociodemográficas y objetivas sobre el tema. Estuvieron presentes los participantes del curso de enfermería de pregrado en una universidad privada. La muestra del estudio consistió en 10 participantes, todos femeninos. Nueve participantes tienen especialización en diferentes áreas y 1 tiene título de máster. Sólo 5 participantes fueron entrenados para actuar en la preceptoría. Todos utilizan metodologías activas en el campo de las prácticas y sólo 6 recibieron capacitación. Se evidó que el enfermero preceptor tiene un papel importante en la formación de futuros profesionales, enfrentando al estudiante como protagonista del aprendizaje. El uso de metodologías activas en las prácticas es relevante, ya que implica los retos existentes en la profesión, desde estructurales hasta pedagógicos.Descriptores: Preceptor, Prácticas Supervisadas, Metodologías Activas.
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