Summary:We describe collection and purification of peripheral blood CD34 ؉ cells from volunteer, normal donors and allogeneic stem cell donors. A total of 98 aphereses were performed on 68 volunteer donors using peripheral venous access. The mean number of nucleated cells collected was 4.6 × 10 10 which included 1.9 × 10 8 CD34 ؉ cells corresponding to 2.7 × ilized PBPCs are now being used in related and unrelated allogeneic transplantation. Apheresis collection is well tolerated and is potentially less invasive than bone marrow harvest. Recently, attention has focused on the number of CD34 + cells in the PBPCs as the component responsible for the time to recovery after intensive chemotherapy and the cells likely responsible for long-term engraftment and reconstitution of hematopoiesis. 9-15 Therefore, an enriched population of CD34 + cells may be a superior donor product. In addition, isolation of CD34 + cells may decrease contaminating tumor cells, 16,17 deplete mature T cells for modulation of graft-versus-host disease [18][19][20] and provide the target of choice for both ex vivo expansion 21,22 and gene transfer. [23][24][25][26] Antibodies directed against the CD34 antigen have provided a means for rapidly purifying and concentrating CD34 + cells using immunomagnetic techniques. 27,28 Although apheresis devices have been shown to be effective for collecting large numbers of nucleated white blood cells, the number of CD34 + cells collected is highly variable depending on factors such as the individual's response to cytokine and the number of blood volumes processed. 29,30 Reliable predictors of CD34 + cell purity, recovery, and clonogenic potential are currently needed and would be advantageous in planning efficient use of growth factor during mobilization and minimizing procedural discomfort to the donor.Although there is a large experience with apheresis from patients on cytotoxic therapeutic regimens, limited data are available from normal volunteers on the safety, efficacy and ability to collect purified CD34 + cells. We present the results of a large series of aphereses (n = 98) and CD34 + cell isolations using the Isolex 300i magnetic separator (n = 30) on G-CSF-mobilized PBPCs from normal volunteers. Adverse events, absolute numbers of cells collected, and CD34 + cell purification are presented. In addition, we present results from 38 CD34 + cell isolations using the Isolex 300i performed on normal allogeneic stem cell donors. These data provide further evidence for the safety and feasibility of collecting adequate numbers of CD34 + cells from the peripheral blood of normal donors for stem cell support, transplantation and gene therapy.
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