As an abundant ROS, hydrogen peroxide (H2 O2 ) plays pivotal roles in plant growth and development. In this work, we conducted for the first time an iTRAQ-based quantitative proteomic analysis of wheat seedling growth under different exogenous H2 O2 treatments. The growth of seedlings and roots was significantly restrained by increased H2 O2 concentration stress. Malondialdehyde, soluble sugar, and proline contents as well as peroxidase activity increased with increasing H2 O2 levels. A total of 3,425 proteins were identified by iTRAQ, of which 157 showed differential expression and 44 were newly identified H2 O2 -responsive proteins. H2 O2 -responsive proteins were mainly involved in stress/defense/detoxification, signal transduction, and carbohydrate metabolism. It is clear that up-regulated expression of signal transduction and stress/defence/detoxification-related proteins under H2 O2 stress, such as plasma membrane intrinsic protein 1, fasciclin-like arabinogalactan protein, and superoxide dismutase, could contribute to H2 O2 tolerance of wheat seedlings. Increased gluconeogenesis (phosphoenol-pyruvate carboxykinase) and decreased pyruvate kinase proteins are potentially related to the higher H2 O2 tolerance of wheat seedlings. A metabolic pathway of wheat seedling growth under H2 O2 stress is presented.
Researchers developed a fuzzy-logic model for predicting the risk of accidents that occur on wet pavements. Preventing wet-pavement accidents has been an extremely difficult and elusive task because they are stochastic events whose occurrence is related to a variety of factors, including vehicle, roadway, human, and environmental characteristics. Conventionally, researchers use linear or nonlinear regression models and probabilistic models to predict wet-pavement accidents. However, these models often are limited in their capability to fully explain the process when the underlying physical system possesses a degree of non-linearity. Therefore, the potential of applying fuzzy logic in this area might be promising. Two fuzzy-logic models were developed and evaluated using accident data and the corresponding traffic data collected from 123 sections of highway in Pennsylvania from 1984 to 1986. The models use skid number, posted speed, average daily traffic, percentage of wet time, and driving difficulty as input variables and the number of wet-pavement accidents as the output variable. The first model is based on Mamdani’s fuzzy-inference method, and the second is a Sugeno-type fuzzy-logic model using the fuzzy-clustering method. The two fuzzy-logic models show superiority over the probabilistic model and the nonlinear regression model. Results indicate that, in addition to predicting the risk of wet-pavement accidents, the fuzzy-logic model can be applied conveniently to determine specific corrective actions that should be undertaken to improve safety.
With recent advances in health systems, the amount of health data is expanding rapidly in various formats. This data originates from many new sources including digital records, mobile devices, and wearable health devices. Big health data offers more opportunities for health data analysis and enhancement of health services via innovative approaches. The objective of this research is to develop a framework to enhance health prediction with the revised fusion node and deep learning paradigms. Fusion node is an information fusion model for constructing prediction systems. Deep learning involves the complex application of machine-learning algorithms, such as Bayesian fusions and neural network, for data extraction and logical inference. Deep learning, combined with information fusion paradigms, can be utilized to provide more comprehensive and reliable predictions from big health data. Based on the proposed framework, an experimental system is developed as an illustration for the framework implementation.
Water quality is highly influenced by the composition and configuration of landscape structure, and regulated by various spatiotemporal factors. Using the Wujiang river watershed as a case study, this research assesses the influence of landscape metrics-including composition and spatial configuration-on river water quality. An understanding of the relationship between landscape metrics and water quality can be used to improve water contamination predictability and provide restoration and management strategies. For this study, eight water quality variables were collected from 32 sampling sites from 2014 through 2017. Water quality variables included nutrient pollutant indicators ammoniacal nitrogen (NH 3 -N), nitrogen (NO 3 − ), and total phosphate (TP), as well as oxygen-consuming organic matter indicators COD (chemical oxygen demand), biochemical oxygen demand (BOD 5 ), dissolved oxygen (DO), and potassium permanganate index (COD Mn ). Partial least squares (PLS) regression was used to quantitatively analyze the influence of landscape metrics on water quality at five buffer zone scales (extending 3, 6, 9, 12, and 15 km from the sample site) in the Wujiang river watershed. Results revealed that water quality is affected by landscape composition, landscape configuration, and precipitation. During the dry season, landscape metrics at both landscape and class levels predicted organic matter at the five buffer zone scales. During the wet season, only class-level landscape metrics predicted water contaminants, including organic matter and nutrients, at the middle three of five buffer scales. We identified the following important indicators of water quality degradation: percent of landscape, edge density, and aggregation index for built-up land; aggregation index for water; CONTAGION; COHESION; and landscape shape index. These results suggest that pollution can be mitigated by reducing natural landscape composition fragmentation, increasing the connectedness of region rivers, and minimizing human disturbance of landscape structures in the watershed area.
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