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| Salmonella organisms are most frequently isolated bacterial agents of food borne outbreaks. Due to its economic importance and risks to human health, salmonellosis poses significant role in public health. Poultry serves as reservoir of Salmonella and poultry meat and eggs have been implicated in food associated outbreaks of salmonellosis. The presence of Salmonella in poultry makes the food chain unsafe from farm to the table. In this review, we explore various public health aspects of food borne salmonellosis originated from poultry. In India, poultry industry is one of the fastest growing segments and the factors linked to salmonellosis in poultry industry are reviewed. The sources and modes of transmission of this organism and the disease symptoms are briefly explained. Various reports of salmonellosis in poultry, with special emphasis on Indian conditions are also discussed. The emergence of multidrug resistant Salmonella is considered as a major global threat to public health, therefore, the antibiotic resistance in Salmonella serovars associated with poultry is also presented.

Background: Mycobacterium group contains several pathogenic bacteria including M. tuberculosis where the emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) is alarming for human and animal health around the world. The condition has further aggravated due to the speed of discovery of the newer drugs has been outpaced by the rate of resistance developed in microorganisms, thus requiring alternative combat strategies. For this purpose, nano-antimicrobials have emerged as a potential option. Objective: The current review is focused on providing a detailed account of nanocarriers like liposome, micelles, dendrimers, solid lipid NPs, niosomes, polymeric nanoparticles, nano-suspensions, nano-emulsion, mesoporous silica and alginate-based drug delivery systems along with the recent updates on developments regarding nanoparticle-based therapeutics, vaccines and diagnostic methods developed or under pipeline with their potential benefits and limitations to combat mycobacterial diseases for their successful eradication from the world in future. Results: Distinct morphology and the underlying mechanism of pathogenesis and resistance development in this group of organisms urge improved and novel methods for the early and efficient diagnosis, treatment and vaccination to eradicate the disease. Recent developments in nanotechnology have the potential to meet both the aspects: nano-materials are proven components of several efficient targeted drug delivery systems and the typical physicochemical properties of several nano-formulations have shown to possess distinct bacteriocidal properties. Along with the therapeutic aspects, nano-vaccines and theranostic applications of nano-formulations have grown in popularity in recent times as an effective alternative means to combat different microbial superbugs. Conclusion: Nanomedicine holds a bright prospect to perform a key role in global tuberculosis elimination program.

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ABSTRACT...... Poultry meat is an excellent source of high quality protein, vitamins and minerals and is not subjected to cultural and religious restrictions. Incorporation of herbal plants and vitamins in the feed of broilers are more acceptable to health conscious consumers. Selenium, an essential micronutrient, has various biological roles in poultry and it is needed for normal growth and maintenance in them. The current study was designed to study the combined effect of Ashwagandha and Selenium on growth performance and carcass traits of broilers. Day old chicks (n=60) were selected and divided in to six groups, with 3 replicates of each group, 20 chicks in each replicate. Ashwagandha and Selenium were supplemented in broiler chicken feed at different levels for different group. The growth performance of broilers were assessed in terms of weekly body weight gain and feed conversion ratio. The carcass quality was analyzed based on dressing percentage, drumstick weight, giblet weight, cooking yield and pH of fresh chicken meat. The data obtained in the present study were analyzed statistically by using Complete Randomized Design one way Anova procedure of SPSS version 20. Average body weight gain of broilers groups treated simultaneously with both the Ashwagandha and Selenium showed significant (P<0.05) difference when compared to other groups. The highest body weight gain was found during the third week of the trial and for the treatment group T 5 . The carcass traits were also found better in Ashwagandha and Selenium treated groups due to the synergistic effect of these dietary inclusions. The current study recommends an inclusion of Ashwagandha and Selenium in the broiler diet at level of 2.5 per cent and 0.20 mg/kg, respectively for the improvement of growth performance and carcass traits in broiler chicken.

Aim:To evaluate the efficacy of recombinant outer membrane proteinC (rOmpC) based enzyme-linked immunoassay (ELISA) for the diagnosis of salmonellosis in poultry.Materials and Methods:Three antigens were prepared, and the indirect ELISA was standardized using the antigens and the antiserum raised in chicken against Omp and rOmpC. Sera were collected from a total of 255 apparently healthy field chickens and screened for the presence of Salmonella antibodies by this ELISA.Results:The sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of Omp revealed major polypeptides at 36, 42 and 52 kDa, and the rOmpC was evident by a single protein band of 43 kDa. The Omp and rOmpC antigen revealed an optimum concentration of 78 and 156 ng, respectively, in the assay, while the whole cell antigen gave an optimum reaction at a concentration of 106 organisms/ml. The test was found to be specific as it did not react with any of the antisera of seven other organisms. The developed ELISA detected Salmonella antibodies from 22 (8.62%) samples with rOmpC antigen, while 24 (9.41%) samples gave a positive reaction with both Omp and whole cell antigens.Conclusion:We suggest rOmpC based indirect ELISA as a suitable screening tool for serological monitoring of poultry flocks.

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