Measurements of turbulence and waves were made as part of the Mesosphere‐Lower Thermosphere Turbulence Experiment (MTeX) on the night of 25–26 January 2015 at Poker Flat Research Range, Chatanika, Alaska (65°N, 147°W). Rocket‐borne ionization gauge measurements revealed turbulence in the 70‐ to 88‐km altitude region with energy dissipation rates between 0.1 and 24 mW/kg with an average value of 2.6 mW/kg. The eddy diffusion coefficient varied between 0.3 and 134 m2/s with an average value of 10 m2/s. Turbulence was detected around mesospheric inversion layers (MILs) in both the topside and bottomside of the MILs. These low levels of turbulence were measured after a minor sudden stratospheric warming when the circulation continued to be disturbed by planetary waves and winds remained weak in the stratosphere and mesosphere. Ground‐based lidar measurements characterized the ensemble of inertia‐gravity waves and monochromatic gravity waves. The ensemble of inertia‐gravity waves had a specific potential energy of 0.8 J/kg over the 40‐ to 50‐km altitude region, one of the lowest values recorded at Chatanika. The turbulence measurements coincided with the overturning of a 2.5‐hr monochromatic gravity wave in a depth of 3 km at 85 km. The energy dissipation rates were estimated to be 3 mW/kg for the ensemble of waves and 18 mW/kg for the monochromatic wave. The MTeX observations reveal low levels of turbulence associated with low levels of gravity wave activity. In the light of other Arctic observations and model studies, these observations suggest that there may be reduced turbulence during disturbed winters.

Though the Kelvin‐Helmholtz instability (KHI) has been extensively observed in the mesosphere, where breaking gravity waves produce the conditions required for instability, little has been done to describe quantitatively this phenomenon in detail in the mesopause and lower thermosphere, which are associated with the long‐lived shears at the base of this statically stable region. Using trimethylaluminum (TMA) released from two sounding rockets launched on 26 January 2018, from Poker Flat Research Range in Alaska, the KHI was observed in great detail above 100 km. Two sets of rocket measurements, made 30 min apart, show strong winds (predominantly meridional and up to 150 ms−1) and large total shears (90 ms−1 km−1). The geomagnetic activity was low in the hours before the launches, confirming that the enhanced shears that triggered the KHI are not a result of the E‐region auroral jets. The four‐dimensional (three‐dimensional plus time) estimation of KHI billow features resulted in a wavelength, eddy diameter, and vertical length scale of 9.8, 5.2, and 3.8 km, respectively, centered at 102‐km altitude. The vertical and horizontal root‐mean‐square velocities measured 29.2 and 42.5 ms−1, respectively. Although the wind structure persisted, the KHI structure changed significantly with time over the interval separating the two launches, being present only in the first launch. The rapid dispersal of the TMA cloud in the instability region was evidence of enhanced turbulent mixing. The analysis of the Reynolds and Froude numbers (Re = 7.2 × 103 and Fr = 0.29, respectively) illustrates the presence of turbulence and weak stratification of the flow.

Long-term monocultures have severely inhibited the cultivation of Chinese peanut (Arachis hypogaea L.). In this study, the effects of continuous cropping on soil chemical properties and microbial communities were investigated in peanut fields that had been in crop rotation for 10 years and in monoculture for 10 years. The results found that long-term monoculture increased the activities of available potassium, available phosphorus, available nitrogen, soil organic matter, urease, acid phosphatase and catalase; while decreasing the activity of catalase. The diversity and abundance of soil bacteria and fungi is higher under continuous peanut cultivation. At the genus level, the relative abundance of potentially beneficial microflora genera was higher in the rhizosphere soil of rotational cropping than in continuous cropping, while the opposite was true for the relative abundance of potentially pathogenic fungal genera. Principal coordinates and cluster analysis indicated that continuous cropping altered the structure of the microbial community. The results of the functional predictions showed significant differences in the functioning of the rhizosphere microbial community between continuous and rotational cropping. In conclusion, long-term continuous cropping changed the chemical properties of the soil, altered the structure and function of the soil bacterial and fungal communities in peanut rhizosphere, which to some extent reduced the relative abundance of potentially beneficial microbial genera and increased the relative abundance of potentially pathogenic fungal genera, thus increasing the potential risk of soil-borne diseases and reducing the yield and quality of peanut. Therefore, in the actual production process, attention should be paid not only to the application of chemical fertilizers, but also to crop rotation and the application of microbial fertilizers.

Plant growth-promoting bacteria (PGPB) can effectively reduce salt damage in plants. Currently, there are many studies on the effects of PGPB on the microbial community structure of rhizosphere soil under salt stress, but fewer studies on the community structure of endophytic bacteria and fungi. We propose that inoculation of endophytic bacteria into the rhizosphere of plants can significantly affect the microbial community structure of the plant’s above-ground and underground parts, which may be the cause of the plant’s “Induced Systemic Tolerance.” The isolated endophytes were re-inoculated into the rhizosphere under salinity stress. We found that, compared with the control group, inoculation with endophytic Bacillus velezensis JC-K3 not only increased the accumulation of wheat biomass, but also increased the content of soluble sugar and chlorophyll in wheat, and reduced the absorption of Na in wheat shoots and leaves. The abundance of bacterial communities in shoots and leaves increased and the abundance of fungal communities decreased after inoculation with JC-K3. The fungal community richness of wheat rhizosphere soil was significantly increased. The diversity of bacterial communities in shoots and leaves increased, and the richness of fungal communities decreased. JC-K3 strain improved wheat’s biomass accumulation ability, osmotic adjustment ability, and ion selective absorption ability. In addition, JC-K3 significantly altered the diversity and abundance of endophytic and rhizosphere microorganisms in wheat. PGPB can effectively reduce plant salt damage. At present, there are many studies on the effect of PGPB on the microbial community structure in rhizosphere soil under salt stress, but there are few studies on the community structure changes of endophytic bacteria and fungi in plants.