Amomum tsao-ko Crevost et Lemarie (A. tsao-ko) is a well-known dietary spice and traditional Chinese medicine. This study aimed to identify the flavonoids in A. tsao-ko and evaluate its antioxidant...
Fabricating a single-chain variable fragment specific for human seminoprotein is very important in antibody-directed enzyme prodrug therapy and NMR imaging for prostate cancer. Here a single-chain Fv specific for gamma-seminoprotein was expressed by RTS. Its activity and the efficiency of entry into prostate cancer cells are investigated by immunoprecipitation and Western blotting and immunofluorescent staining, as well as entry of conjugated magnetic beads into cells. Results showed that ScFv peptides specific for gamma-seminoprotein were successfully prepared, which can bind with the prostate cells specifically and can bring magnetic beads into prostate cancer cells within 15 min, the amount of magnetic beads inside prostate cancer cells increased as the culture time prolonged. ScFv-conjugated magnetic beads did not enter into control cells. In conclusion, the ScFv peptide against human gamma-seminoprotein with biological activity was successfully fabricated, which can take magnetic beads to prostate cancer cells specifically and not to the control cells. This ScFv peptide against human gamma-seminoprotein should be useful in improving the detection and therapy of prostate cancer at early stages and NMR imaging.
This umbrella review is to recapitulate and grade the available evidence of associations between consumption of Zingiberaceae plants/curcumin (Cur) and multiple health‐related outcomes. This study included 161 meta‐analyses of randomized controlled trials in 76 articles with 67 unique health outcomes. Data on heterogeneity and publication bias are considered to assess the quality of evidence. Based on the different impact of Zingiberaceae plants/Cur on human health, the advantages outweigh the disadvantages. Zingiberaceae plants/Cur can mainly improve metabolic syndrome, non‐alcoholic fatty liver disease, cardiovascular disease, and some chronic inflammatory diseases, likewise, obviously relief the pain of osteoarthritis and related diseases. Ginger supplements have been shown to improve vomiting during pregnancy and to relieve nausea and vomiting caused by chemotherapy and surgery. The surgery is any type of surgery, including laparoscopic surgery, gynecological surgery and mixed surgery. Beneficial associations were found with Cur intervention in gastrointestinal, neurological and oral diseases. Zingiberaceae plants/Cur are generally safe and favorable for multiple health outcomes in humans. High‐quality research is further needed to prove the observed associations.
Supplemental Table 1. PRISMA 2020 Checklist. Supplemental Table 2. Search terms and strategy. Supplemental Table 3. PICOS criteria for inclusion and exclusion of studies. Supplemental Table 4. Subgroup analysis of 7 indicators. Supplemental Figure 1. Results of an influence analysis (sensitivity analysis) in which the meta-analysis is re-estimated omitting each long-term study in turn. HDL-C (A), LDL-C (B), TC (C), TG (D). Supplemental Figure 2. Results of an influence analysis (sensitivity analysis) in which the meta-analysis is re-estimated omitting each long-term study in turn. BG (A), HbAlc (B), HOMA-IR (C), insulin (D). Supplemental Figure 3. Results of an influence analysis (sensitivity analysis) in which the meta-analysis is re-estimated omitting each long-term study in turn. DBP (A), SBP (B). Supplemental Figure 4. Funnel plot of the weighted mean difference (WMD) versus the S.E. of the weighted mean difference (WMD) for studies. HDL-C (A), LDL-C (B), TC (C), TG (D). Supplemental Figure 5. Funnel plot of the weighted mean difference (WMD) versus the S.E. of the weighted mean difference (WMD) for studies. BG (A), HbAlc (B), HOMA-IR (C), insulin (D). Supplemental Figure 6. Funnel plot of the weighted mean difference (WMD) versus the S.E. of the weighted mean difference (WMD) for studies. DBP (A), SBP (B).
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