Alpine regions in northern China are the traditional animal husbandry base. The lack of high-quality forage supply resulting from degradation of natural grasslands and low forage production due to short growing seasons greatly restricts development of animal husbandry in these areas. Spring oats have been widely planted in cold regions worldwide harvesting as either grains or forages because of their great adaptative ability to low temperatures and early maturation and high nutritional values. To maximize forage and grain production, we developed a dual-purpose model for spring-sown oats in the cold regions of northeastern China using two oat species, Avena nuda L. (cv. Bayou6) and Avena. sativa L. (cv. Qinghai444). Growth, forage production and quality, grain yield, and re-growth ability of the two oats were investigated in field trials and field demonstration. Maximal dry weight was found to occur at 70 days of emergence for both oats with higher forage production and crude protein (CP) in Bayou6 than Qinghai444 oat species. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) of the two oats increased with time during the early vegetative growing stage, while the relative feed value showed a decrease during vegetative growing stage. The re-growth ability following cutting for the two oats reduced with increasing growth times during vegetative stage. Plant height, tiller density, CP and NDF contents of re-growing seedlings harvested at 30–40 days of emergence did not significantly differ from those of un-cutting control. The overall cumulative dry weight of biomass following cutting at 30 days of emergence was significantly higher than that of control without cutting in both oat species. Seed yield from plots cut at 30 days of emergence for both oat species was insignificantly different from that of control plots. Harvesting of spring-sown oats at 30 days of emergence enhanced forage production, but it did not influence seed yield. Results from 2-year field demonstration confirmed these conclusions. These findings highlight that this dual-purpose oat management model can have great applications in the cold regions of China.
Notch signaling is a highly conserved pathway existed in multicellular organisms. It plays roles in normal human body development, human cancer initiation, progression and metastasis. The Notch negative regulatory region (NRR) is critical for Notch signaling, and cleavage at the S2 site in the NRR ultimately leads to the activation of Notch signaling. To study the function of human NRR1, we expressed the recombinant human NRR1 (rhNRR1) domain in Escherichia coli. After purification, rhNRR1 was obtained with approximately 94% purity according to SDS-PAGE analysis. Furthermore, the polyclonal anti-rhNRR1 serum raised by immunizing mouse with the purified rhNRR1 was able to reduce the generation of active form of Notch1 intracellular domain in HeLa cells, which implied the raised antibody could recognize and bind the natural conformation of Notch1 NRR. Preparation of rhNRR1 by this way is convenient, time-consuming, and could be used to the preparation of anti-NRR1 therapeutic antibody.
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