Robotic weeding enables weed control near or within crop rows automatically, precisely and effectively. A computer‐vision system was developed for detecting crop plants at different growth stages for robotic weed control. Fusion of color images and depth images was investigated as a means of enhancing the detection accuracy of crop plants under conditions of high weed population. In‐field images of broccoli and lettuce were acquired 3–27 days after transplanting with a Kinect v2 sensor. The image processing pipeline included data preprocessing, vegetation pixel segmentation, plant extraction, feature extraction, feature‐based localization refinement, and crop plant classification. For the detection of broccoli and lettuce, the color‐depth fusion algorithm produced high true‐positive detection rates (91.7% and 90.8%, respectively) and low average false discovery rates (1.1% and 4.0%, respectively). Mean absolute localization errors of the crop plant stems were 26.8 and 7.4 mm for broccoli and lettuce, respectively. The fusion of color and depth was proved beneficial to the segmentation of crop plants from background, which improved the average segmentation success rates from 87.2% (depth‐based) and 76.4% (color‐based) to 96.6% for broccoli, and from 74.2% (depth‐based) and 81.2% (color‐based) to 92.4% for lettuce, respectively. The fusion‐based algorithm had reduced performance in detecting crop plants at early growth stages.

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Weed management and control are essential for the production of high-yielding and high-quality crops, and advances in weed control technology have had a huge impact on agricultural productivity. Any effective weed control technology needs to be both robust and adaptable. Robust weed control technology will successfully control weeds in spite of variability in the field conditions. Adaptable weed control technology has the capacity to change its strategy in the context of evolving weed populations, genetics, and climatic conditions. This chapter focuses on key work in the development of robotic weeders, including weed perception systems and weed control mechanisms. Following an extensive introduction, the chapter addresses the challenges of robotic weed control focusing on both perception systems, which can detect and classify weed plants from crop plants, and also weed control mechanisms, covering both chemical and mechanical weed control. A case study of an automated weeding system is provided. Disciplines Agricultural Economics | Agriculture | Bioresource and Agricultural Engineering | Robotics Comments This chapter is published as Steward, Brian, Jingyao Gai, and Lie Tang. "The use of agricultural robots in weed management and control. " In Robotics and automation for improving agriculture, edited by

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Brassinosteroids (BRs) are a group of plant steroid hormones involved in regulating growth, development, and stress responses. Many components of the BR pathway have previously been identified and characterized. However, BR phenotyping experiments are typically performed in a low-throughput manner, such as on Petri plates. Additionally, the BR pathway affects drought responses, but drought experiments are time consuming and difficult to control. To mitigate these issues and increase throughput, we developed the Robotic Assay for Drought (RoAD) system to perform BR and drought response experiments in soil-grown Arabidopsis plants. RoAD is equipped with a robotic arm, a rover, a bench scale, a precisely controlled watering system, an RGB camera, and a laser profilometer. It performs daily weighing, watering, and imaging tasks and is capable of administering BR response assays by watering plants with Propiconazole (PCZ), a BR biosynthesis inhibitor. We developed image processing algorithms for both plant segmentation and phenotypic trait extraction to accurately measure traits including plant area, plant volume, leaf length, and leaf width. We then applied machine learning algorithms that utilize the extracted phenotypic parameters to identify image-derived traits that can distinguish control, drought-treated, and PCZ-treated plants. We carried out PCZ and drought experiments on a set of BR mutants and Arabidopsis accessions with altered BR responses. Finally, we extended the RoAD assays to perform BR response assays using PCZ in Zea mays (maize) plants. This study establishes an automated and non-invasive robotic imaging system as a tool to accurately measure morphological and growth-related traits of Arabidopsis and maize plants in 3D, providing insights into the BR-mediated control of plant growth and stress responses.

Brassinosteroids (BRs) are a group of plant steroid hormones involved in regulating growth, development, and stress responses. Many components of the BR pathway have previously been identified and characterized. However, BR phenotyping experiments are typically performed on petri plates and/or in a lowthroughput manner. Additionally, the BR pathway has extensive crosstalk with drought responses, but drought experiments are time-consuming and difficult to control. Thus, we developed Robotic Assay for Drought (RoAD) to perform BR and drought response experiments in soil-grown Arabidopsis plants. RoAD is equipped with a bench scale, a precisely controlled watering system, an RGB camera, and a laser profilometer. It performs daily weighing, watering, and imaging tasks and is capable of administering BR response assays by watering plants with Propiconazole (PCZ), a BR biosynthesis inhibitor. We developed image processing algorithms for both plant segmentation and phenotypic trait extraction in order to accurately measure traits in 2-dimensional (2D) and 3-dimensional (3D) spaces including plant surface area, leaf length, and leaf width. We then applied machine learning algorithms that utilized the extracted phenotypic parameters to identify image-derived traits that can distinguish control, drought, and PCZ-treated plants. We carried out PCZ and drought experiments on a set of BR mutants and Arabidopsis accessions with altered BR responses. Finally, we extended the RoAD assays to perform BR response assays using PCZ in Zea mays (maize) plants. This study establishes an automated and non-invasive robotic imaging system as a tool to accurately measure morphological and growth-related traits of Arabidopsis and maize plants, providing insights into the BRmediated control of plant growth and stress responses. Arabidopsis thaliana | Zea mays | brassinosteroid | drought | leaf segmentation | phenotypic traits | plant growth | 2D and 3D imaging