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Public spaces are social places that enhance social life and foster social interactions.However, there is a lack of analysis of the patterns of use by people in various types of social relationships. Observational data on four public spaces in Suzhou, China, and Sheffield, UK, assessed users' personal characteristics, activities and spatial occupancies in public spaces.Site factors were not related to personal characteristics, but age and group size were culturally different. Activities and spatial occupancies differed between unaccompanied and accompanied users. Compared to single users, users in groups tended to participate in multiple activities at once, and their activities included more interactions. Single users' spatial distributions conformed to the edge effect, but users in groups were evenly distributed in the spaces. Three types of social relationships were defined: Intimate Pair, Intimate Group and Social Group. Intimate Pairs were most likely to use mobile phones and use private spaces, Intimate Groups were most likely to be talking and sitting and to use park amenities, and Social Groups were most likely to be playing games and relaxing in spacious open areas.Regarding spatial occupancy, Intimate Pairs and Social Groups were most likely to use the middle and peripheral regions, and the Intimate Groups tended to be evenly distributed in the spaces. The results of this study stress the importance of designing public spaces for the types Jingwen Cao& Jian Kang: Cities

The mechanistic target of rapamycin complex 1 (mTORC1) plays a crucial role in controlling cell growth and homeostasis. Deregulation of mTOR signaling is frequently observed in some cancers, making it an attractive drug target for cancer therapy. Although mTORC1 inhibitor rapalog-based therapy has shown positive results in various pre-clinical animal cancer studies, tumors rebound upon treatment discontinuation. Moreover, several recent clinical trials showed that the mTORC1 inhibitors rapamycin and rapalog only reduce the capacity for cell proliferation without promoting cell death, consistent with the concept that rapamycin is cytostatic and reduces disease progression but is not cytotoxic. It is imperative that rapamycin-regulated events and additional targets for more effective drug combinations be identified. Here, we report that rapamycin treatment promotes a compensatory increase in transglutaminase 2 (TGM2) levels in mTORC1-driven tumors. TGM2 inhibition potently sensitizes mTORC1-hyperactive cancer cells to rapamycin treatment, and a rapamycin-induced autophagy blockade inhibits the compensatory TGM2 upregulation. More importantly, tumor regression was observed in MCF-7-xenograft tumor-bearing mice treated with both mTORC1 and TGM2 inhibitors compared with those treated with either a single inhibitor or the vehicle control. These results demonstrate a critical role for the compensatory increase in transglutaminase 2 levels in promoting mTORC1 inhibitor resistance and suggest that rational combination therapy may potentially suppress cancer therapy resistance.

The introduction of the concept of surface properties can help us to better analyze the basic physicochemical property changes of metal−organic framework (MOF) materials before and after fluorine functional group treatment. In this study, several polar and nonpolar probes were selected to determine the surface properties, including surface-dispersive free energy, Lewis acid−base constants of Ni-MOF-74, and perfluoro carboxylic acid-modified Ni-MOF-74-Fn (n = 3, 5, and 7) in the range of 343.15−383.15 K by inverse gas chromatography (IGC). It was observed that the surface energy of the treated Ni-MOF-74-Fn showed a substantial decrease with the growth of the perfluorocarbon alkyl chains and the increase in surface roughness. In addition, Lewis acidic sites exposed by the Ni-MOF-74 material after adopting modification with fluorine functional groups increased with the increase of perfluorinated carboxylic acid chains, and their surface properties changed from amphiphilic acidic to strongly acidic. These results not only enrich the basic physical property data of Ni-MOF-74 but also provide more theoretical basis for the fluorinated functionalized custom-designed MOFs and enrich their applications in the fields of multiphase catalysis, gas adsorption, and chromatographic separation.