Background and Aim Rifampicin is the most common pathogenic factor in anti‐tuberculosis drug‐induced liver injury (AT‐DILI), the mechanisms that it promotes hepatocyte damage in AT‐DILI are not yet to be thoroughly elucidated. In this study, we investigated the potential molecular mechanisms for ferroptosis involving rifampicin hepatotoxicity. Methods Animal and cell injury models of rifampicin were constructed, and the toxicity of rifampicin was assessed by physicochemical staining and cell viability assay. Next, flow cytometry was employed to detect changes in ferroptosis‐related markers, and Western blotting was used to detect protein expression. Then, the important role of autophagy and ferroptosis was verified with small molecule compound intervention. Results We found that ferritinophagy‐induced ferroptosis participates in the toxicity of rifampicin, and the mechanism is that rifampicin precisely activates high‐throughput autophagy, which leads to the massive degradation of ferritin and the increase of free iron. Moreover, rifampicin exhibited conspicuous inhibition of Human 71 kDa heat shock cognate protein (HSPA8) that is intimately associated with Microtubule‐associated protein light chain 3 isoform B (LC3B) expression, in turn, HSPA8 inducer attenuated intracellular autophagy flux. Of note, inducing HSPA8 or inhibition of autophagy and ferroptosis considerably relieved the hepatotoxicity of rifampicin in mouse model. Conclusions The present study highlights the crucial roles of the HSPA8 and autophagy in ferroptotic cell death driving by rifampicin, particularly illumines multiple promising regulatory nodes for therapeutic interventions in diseases involving AT‐DILI.
Human hepatitis B virus (HBV) infection, a primary cause of cirrhosis and liver cancer worldwide, remains a global public health concern due to the associated high morbidity and mortality rates. 1,2 Generally, HBV infection can be controlled by reverse-transcriptase inhibitors (nucleos (t) ide analogs [NAs]) and interferon (IFN) therapy. However, both these medications are available in limited quantities for eliminating HBV. Although antiviral therapy can efficaciously inhibit viral replication and significantly delay disease progression in patients infected with HBV, it is not curative and must be taken for a long period or even a lifetime. 3 Furthermore, once antiviral therapy is discontinued, new intact virus particles can be resynthesized, resulting from the covalently closed circular DNA (cccDNA). 4 Another major cause of the long-term existence of HBV is the dysfunction of the adaptive immune response, and the principal mechanism at play here is the immune tolerance induced by multiple viral antigens (e.g. HBV surface antigen, e antigen, core antigen). 5 Consequently,
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