A high-fat diet and sedentary lifestyle could accelerate aging and hypothalamic inflammation. In order to explore the regulatory mechanisms of lifestyle in the hypothalamus, swimming exercise and diet control were applied in the high-fat diet ApoE-/-mice in our study. 20-week-old ApoE-/-mice fed with 12-week high-fat diet were treated by high-fat diet, diet control and swimming exercise. The results showed that hypothalamic inflammation, glial cells activation and cognition decline were induced by high-fat diet. Compared with the diet control, hypothalamic inflammation, glial cells activation and learning and memory impairment were effectively alleviated by swimming exercise plus diet control, which was related to the increasing expression of SIRT1, inhibiting the expression of NF-κB and raising secretion of GnRH in the hypothalamus. These findings supported the hypothesis that hypothalamic inflammation was susceptible to exercise and diet, which was strongly associated with SIRT1-NF-κB-GnRH expression in the hypothalamus.

Objective. Systematic evaluation of the efficacy of acupuncture combined with cognitive rehabilitation training in the treatment of upper limb spasm after cerebral apoplexy. Methods. The data of CNKI, CBM, CQVIP, Wanfang, and the libraries of Pubmed and Cochrane were searched by computer, and the related literatures about acupuncture combined with cognitive rehabilitation training in the treatment of cognitive dysfunction after stroke were searched. The search time is from January 1, 1995 to January 1, 2022. All data segments were independently analyzed and extracted by two evaluators. After evaluating the quality of the methodology, meta-analysis was carried out by using the RevMan5.4 software. Results. Finally, 11 studies were included, with a total of 789 subjects. The results of meta-analysis indicated that acupuncture combined with cognitive rehabilitation training was superior to simple cognitive rehabilitation training or drugs in the following aspects, the difference exhibited statistically significant, the total effective rate ( RR = 1.58 , 95% CI), latency of P300 ( MD = − 18.46 , 95% CI), amplitude of P300 ( MD = 1.23 , 95% CI (0.82), P < 0.00001 , 95% CI (0.31)), and activity of daily living (ADL), respectively, were significantly higher compared to the control group ( P < 0.00001 ), and the difference was statistically significant ( P < 0.05 ). Based on the results of systematic evaluation, the GRADE system recommendation classification method is used to evaluate the quality of evidence. The results show that the level of evidence is low and the intensity of recommendation is weak. Conclusion. The results of this meta-analysis suggest that the curative effect of acupuncture combined with cognitive rehabilitation training is better compared to simple cognitive rehabilitation training or drugs. However, due to the low quality of the original literature, it needs to be confirmed by multicenter, high-quality, large-sample randomized blind controlled trials in the future.

Context: Alismatis rhizome decoction (AD) exhibits antiatherosclerotic activities. The activity of AD against vascular smooth muscle cell (VSMC) proliferation remains unclear. Objective. The mechanisms and effects of AD on oxidized low-density lipoprotein (ox-LDL)-induced VSMC proliferation were explored. Materials and methods. The male SD rats were fed with AD (2.56 g/mL) or 0.9% NaCl by oral gavage 4 mL twice daily for 7 d. Then, AD-containing serum (ADcs) was collected. MTS assay was applied to measure the VSMC viability. The proliferation of VSMCs was detected by 5-bromodeoxyuridine (BrdU) immunocytochemistry. The microRNA (miRNA) profiling was performed, and the target genes of miRNAs were searched from the TargetScan 7.2 database. The expressions of matrix metalloproteinases-2/9 (MMP-2/9), cyclin D1/E, cyclin-dependent kinase inhibitor 1B (p27), extracellular regulated protein kinases 1/2 (ERK1/2), and ERK1/2 phosphorylation were examined by western blotting or quantitative reverse transcription PCR. Results. The ox-LDL-induced miR-17-92a expression promoted VSMC proliferation. AD and the ERK1/2 inhibitor U0126 (10 μmol/L) inhibited VSMC proliferation and reduced the overexpression of miR-17∼92a. AD was found to inhibit phosphorylation of ERK1/2 and reduced the expression of MMP-2/9 in VSMCs. The expression of cyclin D1/E was suppressed, and p27 was elevated following treatment with AD as well as ERK1/2 inhibitor. According to the TargetScan 7.2 database, the target genes of miR-17∼92a act on tissue inhibitors of metalloproteinases (TIMPs)-MMPs, p27/21 cyclins, and peroxisome-proliferator-activated receptor α (PPARα) ATP-binding cassette transporter (ABC) A1/G1, which are involved in the process of atherosclerosis. Conclusions. AD inhibits ox-LDL-induced VSMC proliferation via inhibiting ERK1/2 and miR-17∼92a activation. The results provide the multitarget mechanisms for application of AD in the treatment of atherosclerosis. It would be helpful to the treatment of cardiovascular and cerebral diseases.

Abstract. In order to make the hotel management work systematic, standardization and automation, achieve the aim of improving the efficiency of hotel guest room management. This paper designs the hotel management system, the overall mission of system development is to make the office staff can quickly and easily complete the hotel guest room management task. From the Angle of actual application to system illustrate the basic situation; And then introduced the system analysis and modeling, focusing on the functional requirements of the hotel management system was analyzed, at the same time on the system design goal, the system use case diagram and so on several aspects, Hotel management system is mainly introduced the three layers of frame structure, and the database design, etc. Application of this system can be flexibly and conveniently on the computer management hotel rooms, thus greatly improves the processing speed, make the management more modern.

Background: Neurovascular unit (NVU) dysfunction is a major process in the pathophysiological process of cerebral ischemia-reperfusion (CI/R). Our previous studies have confirmed that the main active ingredient of Alisma orientale exhibits protective effect on CI/R injury. Therefore, this study is designed to verify whether Alisol A, one of the main active components of Alisma, attenuates CI/R-induced NVU dysfunction through activation of AKT/GSK3β pathway. Methods C57BL/6J mice were used to establish the animal model of global cerebral ischemia- reperfusion (GCI/R) and treated with Alisol A and GSK690693 (an AKT inhibitor). We carried out modified neurological severity score (mNSS) and morris water maze (MWM) to test learning and memory abilities. Nissl staining and CD31, GFAP, Iba1, NeuN immunostaining were performed to determine morphological and quantitative changes in neuronal and endothelial cells, as well as overactivation of astrocytes and microglia. Ultrastructure changes of NVU were observed by transmission electron microscope (TEM). Expression of IL-6, IL-1β, BAX, Bcl-2 and AKT/GSK3βwere detected by enzyme-linked immunosorbent assay (ELISA) and western blot. The alterations in neuronal/glial metabolism were measured using magnetic resonance spectroscopy (MRS). Results The data showed that Alisol A treatment significantly improved neurological deficits and memory loss caused by GCI/R in C57BL/6J mice. Furthermore, Alisol A administration effectively suppressed the disruption of neurons and endothelial cells, activation of astrocytes and microglia, cell apoptosis and release of inflammatory factors in GCI/R-induced C57BL/6J mice, which were reversed by GSK690693. Conclusions Alisol A significantly alleviated NVU damage caused by GCI/R via activation of AKT/GSK3β, suggesting that Alisol A maybe a promising medicine for the treatment of GCI/R.

We present a novel and first-of-its-kind information-theoretic framework for the key design consideration and implementation of a ground-to-unmanned Aerial Vehicle (UAV) (G2U) communication network with an aim to minimize end-to-end transmission delay in the presence of interference in Space-Air-Ground Integrated Networks (SAGIN). To characterize the transmission delay, we utilize Fano’s inequality and derive the tight upper bound for the capacity for the G2U uplink channel in the presence of interference, noise, and potential jamming. In addition, as a function of the location information of the UAV, a tight lower bound on the transmit power is obtained subject to the reliability constraint and the maximum delay threshold. Furthermore, a relay UAV in the dual-hop relay mode, with amplify-and-forward (AF) protocol, is considered, for which we jointly obtain the optimal positions of the relay and the receiver UAVs in the presence of interference, with straight-line, circular, and helical trajectories as UAV tracing. Interestingly, increasing the power gives a negligible gain in terms of delay minimization, though may greatly enhance the outage performance. Moreover, we prove that there exists an optimal height that minimizes the end-to-end transmission delay in the presence of interference. We show the interesting result of the delay analysis. In particular, it is shown that receiver location and the end-to-end signal-to-noise power ratio play a critical role in end-to-end latency. For instance, with the transmitter location fixed to (0,0,0) and the interferer location set to (0, 500 m, 0), the latency generally increases with increasing the receiver’s vertical height (z-axis). With the receiver’s horizontal coordinates, i.e., (xR,yR) set to (0, 0) reducing the receiver’s height from 200 m to 50 m decreases the delay latency (codeword length) by more than 30% for an interference-limited channel. Whereas, for an interference channel with a signal-to-noise power ratio equal to 30 dB, the latency decreases by approximately 2%. The proposed framework can be used in practice by a network controller as a system parameters selection criteria, where among a set of parameters, the parameters leading to the lowest transmission latency can be incorporated into the transmission. The based analysis further set the baseline assessment when applying Command and Control (C2) standards to mission-critical G2U and UAV-to-UAV (U2U) services.

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