A gram-stain-negative, aerobic, ovoid or short rod-shaped, and non-motile strain, designed G7 was isolated from a tidal flat sample collected from the coast of East Sea in Zhoushan, China. Strain G7 grew at 4-40 °C and pH 6.0-9.0 (optimum, 28 °C and pH 7.5) and with 0-7% (w/v) NaCl (optimum, 1%). The predominant respiratory quinone was Q-10 and the major fatty acids (>10%) identified were C ω7c, C and summed feature 3 (C ω7c and/or C ω6c). The polar lipids of strain G7 consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and four unidentified lipids. The genomic DNA G+C content was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G7 formed a distinct lineage belonging to the Roseobacter clade of the family Rhodobacteraceae. On the basis of morphological, physiological, and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain G7 is described as a novel species in a new genus, for which the name Aestuarium zhoushanense gen. nov., sp. nov. (type strain G7 = MCCC 1K03229 = KCTC 52584) is proposed.
A Gram-stain-negative, aerobic, rod-shaped bacterial strain, designated ZYSR67-Z, was isolated from a pharmaceutical wastewater sample collected from a chemical factory in Zhejiang, China. The strain was motile by a single polar flagellum and grew at 4-42 °C (optimum, 35 °C), pH 5.0-9.0 (optimum, 6.0) and 0-5.0% (w/v) NaCl (optimum, 2.0%). Based on multilocus sequence analysis using 16S rRNA, gyrB, rpoB and rpoD, the strain ZYSR67-Z formed a distinct phylogenetic group in the genus Pseudomonas. The average nucleotide identity values between strain ZYSR67-Z and the closely related 10 type strains of the Pseudomonas species were 75.8-78.6%. The in silico DNA-DNA hybridization values indicated that strain ZYSR67-Z and the type strains of the Pseudomonas shared 21.4-23.1% DNA relatedness. The predominant isoprenoid quinone system was ubiquinone-9 while ubiquinone-8 was present in trace amounts. The major fatty acids (> 10%) identified were C, C, C ω7c and summed features 3 (C ω7c and/or iso-C 2OH). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content was 62.6 mol%. On the basis of morphological, physiological and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain ZYSR67-Z was proposed to represent a novel species of the genus Pseudomonas, named Pseudomonas pharmafabricae sp. nov.. The type strain is ZYSR67-Z (= CGMCC 1.15498 = JCM 31306).
To investigate the purification effect of the new adsorption material containing bioreactor and the critical role of non-culture (VBNC) bacteria in eutrophication ecosystem, major water quality parameters and microbial community were determined and analyzed in prepared eutrophic water for 2 years monitoring. The results showed that removal rates of total phosphorus (TP), total nitrogen (TN), chlorophyll-a (Chl-a) and chemical oxygen demand (COD) ranged of 90.7% -95.9%, 84.5% -92.4%, 87.9% -95.8% and 68.3% -82.7%, respectively, indicating the designed bioreactor possessed high efficiency in eutrophic water treatment. Although the bioreactor had been operated for 2 years, water from treatment group was more clearer and odorless than control group, exhibiting the long service life of the bioreactor. Stopping operation in August caused the removal rates of major water quality parameters significantly decrease (p < 0.05), revealing stopping operation and high temperature in Summer exerted dual effect on the bioreactor, whereas the impact could be minimized when the bioreactor was running. According to most probable number (MPN) method, the total bacteria under +Rpf treatment were higher than under -Rpf treatment, implying Rpf could resuscitate VBNC bacteria in eutrophication ecosystem. Nine VBNC bacteria were isolated based on the BLAST results of 16S rRNA gene, and these bacteria might contribute to eutrophic water treatment based on their functions, such as phosphorus-collecting and denitrification. Those results provided new insights for engineering technology innovation and had benefit in eutrophic water treatment.
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