Ice structuring collagen peptides (ISCPs) from porcine skin collagen hydrolysates were isolated by an affinity adsorption system, and then its antifreeze and cryoprotection activity, ice-binding mechanism and making performance of steamed bread were investigated. The results showed ISCPs with maximal thermal hysteresis activity (2.768C) were obtained with a collagen hydrolysate concentration of 1 mg/mL, adsorption time of 10 hr and adsorption temperature of 258C during the second round of adsorption. After purification, MS spectrum analyzed one of the ISCPs and inferred to be GLLGPLGPRGLL with a molecular weight of 1163.8 Da. The binding of ISCP to ice could be attributed to the structural match and hydrogen bond formation between ice surface and the ISCP.Compared with the control group, the baking and textural characteristics of frozen dough were significantly improved by ISCP at an addition concentration of 0.3% (wt/vol) after 4 weeks frozen storage.
Practical applicationsPorcine skin represents a problematic by-product in meat manufacturing industry due to its high pollutant load, and it is usually discarded as waste or used for low value purposes. In order to find potential applications for this by-product, ISCPs from porcine skin hydrolysates were isolated by an affinity adsorption system. The result demonstrated that ISCPs exhibited a strong antifreeze and cryoprotection activity, and had a potential application in food preservation and cryoprotection of cells and tissues.
K E Y W O R D Scryoprotection activity, frozen dough, ice affinity adsorption system, ice-binding interaction, ice structuring collagen peptides
A capillary tube air-conditioning system was designed after presenting the design method of the cold and heat source, water system, fresh air system, control system. In the paper, the experimental method was employed to study the change and distribution of indoor-air temperature, and the response of capillary tube mat surface temperature, while the computational method was employed to analysis the tube mat radiant heat transfer and convective heat transfer. It is concluded that, the indoor temperature rose faster and the uniformity of indoor temperature distribution was better when ceiling radiant heating of capillary tube was adopted.
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