CARS holography captures both the amplitude and the phase of a complex anti-Stokes field, and can perform three-dimensional imaging by digitally focusing onto different depths inside a specimen. The application of CARS holography for bio-imaging is demonstrated. It is shown that holographic CARS imaging of sub-cellular components in live HeLa cells can be achieved.
In recent years, we and others have developed a type of reflective tomography based on coherent processing of Doppler frequency-shifted echoes from rotating objects. The objects are placed in the Fraunhofer region and irradiated with microwaves as they rotate. The returning signals are sampled and stored in digital form.These are later processed using an optical computer, and highly-resolved, two-dimensional images (tomograms) can be reconstructed.Holographic character is apparent in the processed data.To produce an image, we first make a data film strip and then irradiate it with light from a He -Ne laser in our coherent optical processor.The available resolution predicts images comparable to those produced with digital computation. A coherent optical system is thus able to provide effective and cost -efficient parallel processing in reconstructing coherent Doppler tomograms.
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