Diversity-oriented biosynthesis of a library of antimycin-like compounds (380 altogether) was accomplished by using multiplex combinatorial biosynthesis. The core strategy depends on the use of combinatorial chemistry at different biosynthetic stages. This approach is applicable for the diversification of polyketides, nonribosomal peptides, and the hybrids that share a similar biosynthetic logic.

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Seam-cutting and seam-driven techniques have been proven effective for handling imperfect image series in image stitching. Generally, seam-driven is to utilize seam-cutting to find a best seam from one or finite alignment hypotheses based on a predefined seam quality metric. However, the quality metrics in most methods are defined to measure the average performance of the pixels on the seam without considering the relevance and variance among them. This may cause that the seam with the minimal measure is not optimal (perception-inconsistent) in human perception. In this paper, we propose a novel coarse-tofine seam estimation method which applies the evaluation in a different way. For pixels on the seam, we develop a patch-point evaluation algorithm concentrating more on the correlation and variation of them. The evaluations are then used to recalculate the difference map of the overlapping region and reestimate a stitching seam. This evaluation-reestimation procedure iterates until the current seam changes negligibly comparing with the previous seams. Experiments show that our proposed method can finally find a nearly perception-consistent seam after several iterations, which outperforms the conventional seam-cutting and other seam-driven methods.

BackgroundHigh production cost of bioplastics polyhydroxyalkanoates (PHA) is a major obstacle to replace traditional petro-based plastics. To address the challenges, strategies towards upstream metabolic engineering and downstream fermentation optimizations have been continuously pursued. Given that the feedstocks especially carbon sources account up to a large portion of the production cost, it is of great importance to explore low cost substrates to manufacture PHA economically.ResultsEscherichia coli was metabolically engineered to synthesize poly-3-hydroxybutyrate (P3HB), poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P3HB4HB), and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using acetate as a main carbon source. Overexpression of phosphotransacetylase/acetate kinase pathway was shown to be an effective strategy for improving acetate assimilation and biopolymer production. The recombinant strain overexpressing phosphotransacetylase/acetate kinase and P3HB synthesis operon produced 1.27 g/L P3HB when grown on minimal medium supplemented with 10 g/L yeast extract and 5 g/L acetate in shake flask cultures. Further introduction succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, and CoA transferase lead to the accumulation of P3HB4HB, reaching a titer of 1.71 g/L with a 4-hydroxybutyrate monomer content of 5.79 mol%. When 1 g/L of α-ketoglutarate or citrate was added to the medium, P3HB4HB titer increased to 1.99 and 2.15 g/L, respectively. To achieve PHBV synthesis, acetate and propionate were simultaneously supplied and propionyl-CoA transferase was overexpressed to provide 3-hydroxyvalerate precursor. The resulting strain produced 0.33 g/L PHBV with a 3-hydroxyvalerate monomer content of 6.58 mol%. Further overexpression of propionate permease improved PHBV titer and 3-hydroxyvalerate monomer content to 1.09 g/L and 10.37 mol%, respectively.ConclusionsThe application of acetate as carbon source for microbial fermentation could reduce the consumption of food and agro-based renewable bioresources for biorefineries. Our proposed metabolic engineering strategies illustrate the feasibility for producing polyhydroxyalkanoates using acetate as a main carbon source. Overall, as an abundant and renewable resource, acetate would be developed into a cost-effective feedstock to achieve low cost production of chemicals, materials, and biofuels.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0949-0) contains supplementary material, which is available to authorized users.

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