Abstract. A set of rapid near infrared spectroscopy analysis methods were developed for the content determination of Polygonum perfoliatum L.. Investigate the relation between climate and proportion of the active ingredient content. Models were established with partial least squares (PLS) algorithm. The correlation coefficients between predicted value and measured value of the three calibration models are above 0.9 which indicated that there have no differences between NIR and UV in content determination. NIR has a broad prospect of application in the quality control of Chinese traditional medicine. Proportion of polyphenol and flavone is from 1.71 to 2.26. Combined with the local climate analysis shows: Tropical or subtropical humid monsoon climate conducive to the accumulation of polyphenol ingredient, large temperature difference between day and night conducive to the accumulation of flavone ingredient. The result can provide reference for the cropping of Polygonum perfoliatumL.. IntroductionPolygonum perfoliatum L. (PPL) is the aerial parts of the polygonaceae plant which was recorded in "all diseases rejuvenation" written by Tingxian Gong in Ming Dynasty(1587) [1] . Its nature and flavors are plain, sour and bitter. It can induce diuresis for removing edema, cleanup heat detoxifcation, relieve a cough. Modern pharmacological studies have shown that PPL have activities in anti-inflammatory and anti-tumor effect. Owing to its curative effects, it had been recorded in the 2010 and 2015 edition of the Chinese pharmacopoeia (ChP) as a new variety which is payed extensive attentionin in recent years [2] . PPL is widely distributed in China. Accordingly, the quality of the herbal raw materials fluctuates, owing to varying ecological environments [3][4][5] . For the efficacy of Traditional Chinese Medicine (TCM) are based on a certain ratio of multi-component, so study the ratio of active components and its relationship with climate are very important [6,7] . Near-infrared spectroscopy (NIR) technology combined with chemometric can directly analysis of multi-components in a few seconds no matter the opaque samples are granulated, paste or solid and needn't pretreated of the sample. AS a high-speed, non-destructive technique, it has showed vast prospect in TCM research [8][9][10][11][12] . This study aimed to establish a fast determination of multi-component method by NIR. Materials and Methods Equipment and MaterialsNIR analyzer (Nicolet 6700, Thermo Scientific) with an InGaAs detector was used for NIR spectra collection and analysis; UV-visible spectrophotometer (UV 1200, Shanghai Precision & Scientific Instrument Co., Ltd.) was used to detect the content of PPL; gallic acid (110831-200302, Sample preparationThe samples were milled into powder with a grinder. The final powder samples were prepared by passing the ground powder through a 50-mesh sieve. To ensure that moisture was not an interfering factor, all samples were dried in an oven at 40°C for 12 h, placed in the dryer alternate. NIR spectra collection5g po...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.