Abstract. A post-column reactor was designed for chemiluminescence detection in the capillary electrophoresis separation of isoluminol thiocarbamyl derivatives of amino acids. This system was characterized with respect to hydrogen peroxide concentration and flow rate, surfactant concentration, microperoxidase concentration, and mixing distance. A linear calibration curve (R > 0.999) was COnStNCted for isoluminol ranging from at least 30 femtomoles to the detection limit of 40 attomoles injected; the dynamic range of the instrument is at least three orders of magnitude. Detection limit for the isoluminol thiocarbamyl derivative of valine was 500 attomoles. The theoretical plate counts for the raw data are typically 1 x 1 6 for labeled amino acids, and a factor of two better for isoluminol.

Amidea 1 wcrr \ynthesired by thc reaction sequence: Br(CH,).COCI + Br(CH,),,CONHPhNO, -+ [C,,H,,N+(CH,),(CH,),CONHPhNO~] Br-. After recr)stalIiratioii from EtOAcCH,CN l a had a melting point of 208-209 "C and I b melted at 156-1.57 C . Gitionic substrate 2 was obtained as a gel-like, yellow solid from N-methyl-/~-nitroaniline according to the Scheme for 1 above. All compounds had ' H and "C NMR spectra and elemental analysis consistent with given formulae. Kinetic data were obtained by following the absorbance of reaction mixtures containing surfactant components at 390 nm (Table 1). All UViVis data were obtained with a Hewlett-Packard Model 8450 spectrophotometer. Mixture components were added to the cuvet by pipet and shaken vigorously by hand for 2-3 s.The results were not influenced by the order of addition of mixture components.

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hid 1994, 37. 297662080. Der Ester 7-[(ni-Azido-o-nitro)benzoyl]taxol wurde ebenfalls als Photoartinitdtsdnalogon genutzt: J. M. Carhoni. V. Farina, S.