A pH 4.0 buffered solution of the fluorochrome acridine orange was used to stain samples of 2,704 blood cultures that failed to yield visible evidence of growth after 1 day of incubation. Results obtained by the staining method were compared with those obtained by aerobic and anaerobic subcultures simultaneously performed upon the same cultures. Of the 109 culture-positive blood specimens initially detected by the acridine orange and the subculture methods, 85 (78%) were detected by both acridine orange and subculture techniques, 14 (12.8%) were detected by subculture alone, and 10 (9.2%) were detected by acridine orange alone. The differences between the subculture and acridine orange methods were not found to be statistically significant (P > 0.1). The acridine orange method represents a rapid and inexpensive alternative to conventional subculture techniques for the detection of bacteria in blood cultures that fail to yield visible evidence of growth after 1 day of incubation.
Determination of the effectiveness of chemical treatments for Herpes simplex type 2 infections has generally relied on a reduction in the rate of mortality. A more natural progression of the disease is seen when non-progressive cutaneous lesions are induced. Treatment of characteristic cutaneous lesions with ultrasound alone, a cream compound designated Herpigon alone, or a combination of ultrasound and Herpigon were compared to determine the time required for lesions to heal and the amount of virus recoverable from treated lesions. Results indicate that the use of Herpigon alone or in combination with ultrasound resulted in a significant reduction in the time required for the lesions to heal. A comparison of the amount of virus present in treated lesions indicated that the combined treatment was most effective in reducing the amount of recoverable virus.
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