Citrus target spot disease caused by Pseudofabraea citricarpa, which poses a major threat to citrus production worldwide. Here, we report a high-quality genome assembly and annotation of a strain isolated from Chongqing, China. We have provided valuable insights into the evolution and pathogenicity of this fungus. The genome of P. citricarpa spans of 44,005,176 bp with a G+C content of 44.71%, and its coding genes have been extensively annotated in various databases such as GO and KEGG. Notably, we have predicted important proteins, such as secreted proteins, transmembrane proteins, and carbohydrate active enzymes. Our findings serve as a key resource for understanding the global spread of this pathogen, enabling better control measures to be implemented. In conclusion, this study enriches our understanding of P. citricarpa and provides a foundation for future research in the field.
In recent years, a kind of mulberry pests which were known as mulberry pyralid Diaghania pyloalis in different sericultural areas occurred popularly in China. This study aims to screening high efficacy and low toxicity insecticides for controlling Diaghania pyloalis. The current study can help understand integrated pest management (IPM) of Diaghania pyloalis by scientific and reasonable insecticide use. Field experiment was carried out to investigate five insecticides, treated with five concentration gradients. The result shows that Imidacloprid and Chlorfenapyr had higher control effect on Diaghania pyloalis, which was relatively safe to the silkworm. The silkworm was fed until cocooning in the treatment area which sprayed for 15 days and 30 days, investigated cocoon quality achievement, the result shows that 10% Imidacloprid WP and 8% Chlorfenapyr ME had no toxicity to silkworm larvae, there was no significant difference between the economic property index of silkworm in treatment areas and that of the control area. To control Diaghania pyloalis, 2000 dilution of 10% Imidacloprid WP and 8% Chlorfenapyr ME can be used in sericultural production, the leaves should be picked at intervals of 15 days to avoid affecting the quality of cocoons.
Mulberry sclerotial disease is a destructive disease that afflicts mulberry fruits throughout the world. In recent years, the impact of mulberry sclerotial disease has increased with the development of the mulberry fruit industry. Ciboria carunculoides, C. shiraiana, and Scleromitrula shiraiana are all pathogens causing mulberry sclerotial disease, but the dominant pathogen may be different in different mulberry planting areas. In this study, we investigated the causal agents of mulberry sclerotial disease in Chongqing and Sichuan, the main mulberry planting areas in south‐west China. The results showed that C. carunculoides was the dominant pathogen in the investigated areas, and the disease incidence rate on some widely cultivated mulberry varieties exceeded 90%. Based on the differences in internal transcriber spacer sequences of different pathogens, we developed a method using cleaved amplified polymorphic sequences (CAPS) from digestion with the three restriction enzymes HindIII, EcoRI, and MluI for rapid detection of pathogens on mulberry fruits. Use of this method confirmed that the diseased fruits of some mulberry varieties were infected by a mixture of C. carunculoides and S. shiraiana. Long‐fruit mulberry, which was considered to be resistant to sclerotial disease in the past, was also infected by C. carunculoides. Diseased fruit infected by C. shiraiana or by S. shiraiana alone were not detected. Our results suggest that C. carunculoides is probably the dominant pathogen causing mulberry sclerotial disease in south‐west China. The CAPS method for rapid detection of pathogens could assist diagnosis, epidemiology, and disease monitoring.
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