In this study, we have developed a mild and effective method to prepare a metal-organic framework (MOF)-based microcapsule by the self-assembly of pre-synthesized zeolite imidazolate framework-8 (ZIF-8) nanoparticles at the oil-water interface combined with deposition of a dense ZIF-8 coating outside the capsule. By introducing the enzyme Candida antarctica lipase B (CalB) directly into the stabilizer ZIF-8 or the water phase of Pickering emulsion during the preparation process, we achieved that the enzyme was immobilized within the shell (CalB@ZIF-8@cap) or in the cavity (ZIF-8@cap-CalB) of the microcapsules, respectively. The resulting CalB-loaded microcapsules were robust and had a core-shell structure proved by scanning electron microscopy. Meanwhile, Fourier transform infrared spectroscopy was conducted to confirm the encapsulation of enzymes in the microcapsules and their position in the microcapsules was confirmed by fluorescence microscopy. Furthermore, through the comparison of transesterification reactions between a pair of small substrates and a pair of larger ones, the two types of CalB-loaded microcapsules showed great catalytic activity, stability and size selectivity, and the catalytic activity of CalB@ZIF-8@cap was slightly higher than that of ZIF-8@cap-CalB. Importantly, due to the large size of the microcapsules, the catalyst could be separated from the reaction system by sedimentation, thereby reducing the energy consumption for separation. These kinds of multifunctional MOF-enzyme composites may open up new opportunities for the biocatalysis and microreactor.
As computer technology advanced, VR has emerged. It is a virtual environment based on high-tech computer technology that can bring people a sense of reality and sensory experience. Through specific devices or equipment, it uses unique means to interact and communicate with the target object in the virtual environment, so that the professor can get a sense of reality and immersive experience. In order to increase students’ interest in teaching content, and at the same time to develop the education industry to achieve technology, this article combines virtual reality technology with experimental teaching. The paper discusses the use of VR in animation art experimental teaching and plans to provide thoughts and instructions about the fresh way in animation experimental teaching. This paper proposes the application research methods of VR in animation experiment teaching, including literature research method, expert interview method, questionnaire survey method, VR animation art teaching based on image method, and VR animation teaching evaluation clustering algorithm, which is used for conducting research experiments on the utilization of VR in animation art experimental learning. The experimental results in this article show that 96.25% of students like animation art VR experimental teaching system, which is helpful to the development of the teaching work.
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