Abstract. The main advancements of the Beijing Climate Center (BCC) climate system model from phase 5 of the Coupled Model Intercomparison Project (CMIP5) to phase 6 (CMIP6) are presented, in terms of physical parameterizations and model performance. BCC-CSM1.1 and BCC-CSM1.1m are the two models involved in CMIP5, whereas BCC-CSM2-MR, BCC-CSM2-HR, and BCC-ESM1.0 are the three models configured for CMIP6. Historical simulations from 1851 to 2014 from BCC-CSM2-MR (CMIP6) and from 1851 to 2005 from BCC-CSM1.1m (CMIP5) are used for models assessment. The evaluation matrices include the following: (a) the energy budget at top-of-atmosphere; (b) surface air temperature, precipitation, and atmospheric circulation for the global and East Asia regions; (c) the sea surface temperature (SST) in the tropical Pacific; (d) sea-ice extent and thickness and Atlantic Meridional Overturning Circulation (AMOC); and (e) climate variations at different timescales, such as the global warming trend in the 20th century, the stratospheric quasi-biennial oscillation (QBO), the Madden–Julian Oscillation (MJO), and the diurnal cycle of precipitation. Compared with BCC-CSM1.1m, BCC-CSM2-MR shows significant improvements in many aspects including the tropospheric air temperature and circulation at global and regional scales in East Asia and climate variability at different timescales, such as the QBO, the MJO, the diurnal cycle of precipitation, interannual variations of SST in the equatorial Pacific, and the long-term trend of surface air temperature.
A metagenomic cosmid library was prepared in Escherichia coli from DNA extracted from the contents of rabbit cecum and screened for cellulase activities. Eleven independent clones expressing cellulase activities (four endo-beta-1,4-glucanases and seven beta-glucosidases) were isolated. Subcloning and sequencing analysis of these clones identified 11 cellulase genes; the encoded products of which shared less than 50% identities and 70% similarities to cellulases in the databases. All four endo-beta-1,4-glucanases and all seven beta-glucosidases, respectively, belonged to glycosyl hydrolase family 5 (GHF 5) and family 3 (GHF 3) and formed two separate branches in the phylogenetic tree. Ten of the 11 cloned cellulases exhibited highest activities at pH 5.5 approximately 7.0 and 40 approximately 55 degrees C, a condition similar to that in the rabbit cecum. All the four endo-beta-1,4-glucanases could hydrolyze a wide range of beta-1,4-, beta-1,4/beta-1,3- or beta-1,3/beta-1,6-linked polysaccharides. One endo-beta-1, 4-glucanase gene, umcel5G, was overexpressed in E. coli, and the purified recombinant enzyme was characterized in detail. The enzymes cloned in this work represented at least some of the cellulases operating efficiently in the rabbit cecum. This work provides the first snapshot on the cellulases produced by bacteria in rabbit cecum.
[1] The paper examines terrestrial and oceanic carbon budgets from preindustrial time to present day in the version of Beijing Climate Center Climate System Model (BCC_CSM1.1) which is a global fully coupled climate-carbon cycle model. Atmospheric CO 2 concentration is calculated from a prognostic equation taking into account global anthropogenic CO 2 emissions and the interactive CO 2 exchanges of land-atmosphere and ocean-atmosphere. When forced by prescribed historical emissions of CO 2 from combustion of fossil fuels and land use change, BCC_CSM1.1 can reproduce the trends of observed atmospheric CO 2 concentration and global surface air temperature from 1850 to 2005. Simulated interannual variability and long-term trend of global carbon sources and sinks and their spatial patterns generally agree with other model estimates and observations, which shows the following: (1) Both land and ocean in the last century act as net carbon sinks. The ability of carbon uptake by land and ocean is enhanced at the end of last century. (2) Interannual variability of the global atmospheric CO 2 concentration is closely correlated with the El Niño-Southern Oscillation cycle, in agreement with observations. (3) Interannual variation of the land-to-atmosphere net carbon flux is positively correlated with surface air temperature while negatively correlated with soil moisture over low and midlatitudes. The relative contribution of soil moisture to the interannual variation of land-atmosphere CO 2 exchange is more important than that of air temperature over tropical regions, while surface air temperature is more important than soil moisture over other regions of the globe.Citation: Wu, T., et al. (2013), Global carbon budgets simulated by the Beijing Climate Center Climate System Model for the last century,
influence of exaggerated IOD variability in the model. The results imply that the upper limit of intraseasonal predictability is modulated by large-scale external forcing background state in the tropical Indian Ocean. Two additional sets of hindcast experiments with improved atmosphere and ocean initial conditions (referred to as S2S_IEXP1 and S2S_IEXP2, respectively) are carried out, and the results show that the overall MJO forecast skill is increased to 21-22 days. It is found that the optimization of initial sea surface temperature condition largely accounts for the increase of the overall MJO forecast skill, even though the improved initial atmosphere conditions also play a role. For the DYNAMO/CINDY field campaign period, the forecast skill increases to 27 days in S2S_IEXP2. Nevertheless, even with improved initialization, it is still difficult for the model to predict MJO propagation across the western hemisphere-western Indian Ocean area and across the eastern Indian Ocean-Maritime Continent area. Especially, MJO prediction is apparently limited by various interrelated deficiencies (e.g., overestimated IOD, shorter-than-observed MJO life cycle, Maritime Continent prediction barrier), due possibly to the model bias in the background moisture field over the eastern Indian Ocean and Maritime Continent. Thus, more efforts are needed to correct the deficiency in model physics in this region, in order to overcome the wellknown Maritime Continent predictability barrier.
Them2 (thioesterase superfamily member 2) is a 140-aminoacid protein of unknown biological function that comprises a single hotdog fold thioesterase domain. On the basis of its putative association with mitochondria, accentuated expression in oxidative tissues and interaction with StarD2 (also known as phosphatidylcholine-transfer protein, PC-TP), a regulator of fatty acid metabolism, we explored whether Them2 functions as a physiologically relevant fatty acyl-CoA thioesterase. In solution, Them2 formed a stable homotetramer, which denatured in a single transition at 59.3 °C. Them2 exhibited thioesterase activity for medium- and long-chain acyl-CoAs, with Km values that decreased exponentially as a function of increasing acyl chain length. Steady-state kinetic parameters for Them2 were characteristic of long-chain mammalian acyl-CoA thioesterases, with minimal values of Km and maximal values of kcat/Km observed for myristoyl-CoA and palmitoyl-CoA. For these acyl-CoAs, substrate inhibition was observed when concentrations approached their critical micellar concentrations. The acyl-CoA thioesterase activity of Them2 was optimized at physiological temperature, ionic strength and pH. For both myristoyl-CoA and palmitoyl-CoA, the addition of StarD2 increased the kcat of Them2. Enzymatic activity was decreased by the addition of phosphatidic acid/phosphatidylcholine small unilamellar vesicles. Them2 expression, which was most pronounced in mouse heart, was associated with mitochondria and was induced by activation of PPARα (peroxisome-proliferator-activated receptor α). We conclude that, under biological conditions, Them2 probably functions as a homotetrameric long-chain acyl-CoA thioesterase. Accordingly, Them2 has been designated as the 13th member of the mammalian acyl-CoA thioesterase family, Acot13.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.