Historically, it has been difficult to propagate cells in vitro that are derived directly from human tumors or healthy tissue. However, in vitro preclinical models are essential tools for both the study of basic cancer biology and the promotion of translational research, including drug discovery and drug target identification. This protocol describes conditional reprogramming (CR), which involves coculture of irradiated mouse fibroblast feeder cells with normal and tumor human epithelial cells in the presence of a Rho kinase inhibitor (Y-27632). CR cells can be used for various applications, including regenerative medicine, drug sensitivity testing, gene expression profiling and xenograft studies. The method requires a pathologist to differentiate healthy tissue from tumor tissue, and basic tissue culture skills. The protocol can be used with cells derived from both fresh and cryopreserved tissue samples. As approximately 1 million cells can be generated in 7 d, the technique is directly applicable to diagnostic and predictive medicine. Moreover, the epithelial cells can be propagated indefinitely in vitro, yet retain the capacity to become fully differentiated when placed into conditions that mimic their natural environment.

The experimental results of low pressure supersonic molecular beam injection (SMBI) fuelling on the HL-2A closed divertor indicate that during the period of pulsed SMBI the power density convected at the target plate surfaces was 0.4 times of that before or after the beam injection. An empirical scaling law used for the SMBI penetration depth for the HL-2A plasma was obtained. The cluster jet injection (CJI) is a new fuelling method which is based on and developed from the experiments of SMBI in the HL-1M tokamak. The hydrogen clusters are produced at liquid nitrogen temperature in a supersonic adiabatic expansion of moderate backing pressure gases into vacuum through a Laval nozzle and are measured by Rayleigh scattering. The measurement results have shown that the averaged cluster size of as large as hundreds of atoms was found at the backing pressures of more than 0.1 MPa. Multifold diagnostics gave coincidental evidence that when there was hydrogen CJI in the HL-2A plasma, a great deal of particles from the jet were deposited at a terminal area rather than uniformly ablated along the injecting path. SMB with clusters, which are like micro-pellets, will be of benefit for deeper fuelling, and its injection behaviour was somewhat similar to that of pellet injection. Both the particle penetration depth and the fuelling efficiency of the CJI were distinctly better than that of the normal SMBI under similar discharge operation. During hydrogen CJI or high-pressure SMBI, a combination of collision and radiative stopping forced the runaway electrons to cool down to thermal velocity due to such a massive fuelling.

Oblique droplet impacts onto a smooth surface at various inclination angles and at different ambient gas pressures were investigated using high-speed photography. It was found that the droplet splash can be entirely suppressed either by increasing the inclination angle or by reducing the ambient pressure. Variations of the threshold angle required for the splash suppression as a function of the impact velocity were determined, as well as the threshold pressure as a function of the inclination angle and the impact velocity. The threshold pressure increases monotonically as the inclination angle increases for small enough impact velocities but varies in a nonmonotonic manner for high enough impact velocities. Modifications of the existing splash model permit the theoretical determination of the splash threshold conditions that agree well with the experimental observations. It is shown that it is the velocity of the lamella tip that determines the splash onset.

The HL-2A tokamak has a very closed divertor geometry, and a new infrared camera has been installed for high resolution studies of edge-localized mode (ELM) heat load onto the outer divertor targets. The characteristics of power deposition patterns on the lower outer divertor target plates during ELMs are systematically analysed with infrared thermography. The ELM energy loss is in the range of 3%–8% of the total plasma stored energy. The peak heat flux on the outer divertor targets during ELMs currently achieved in HL-2A is about 1.5–3.2 MW m−2, the wetted area is about 0.5–0.7 m2, and the corresponding integrated power decay length at the midplane is about 25–40 mm. The rise time of the ELM power deposition is in the range of about 100 μs to 400 μs, and the decay time is typically 1.5 to 4 times longer than the corresponding rise time. Convective transport along open field lines during the ELM rise phase from the midplane towards the divertor targets is implied due to the correlation of parallel transport time in the scrape-off layer (SOL) and ELM power rise time. The peak ELM energy fluence is compared with those predicted by models and with experimental data from JET, ASDEX Upgrade, MAST, and COMPASS. The results, as a whole, show a good agreement.

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