Shrimp is one of the most important food allergens. Tropomyosin is its major allergen. Wherein Pen a 1, contains five antibody binding regions, has been identified as the only major shrimp allergen. However, the study on IgE with response to shrimp allergen is still a serious lack, compared with the allergenic proteins. Particularly in the aspects of the preparation of IgE in vitro, it is restricted and can only obtain the complete IgE molecules by polyclonal or monoclonal technology. As for the preparation of small molecule IgE to the shrimp allergen has not yet been reported. This study attempts to carry out research on obtaining of cell materials that are used to clone. It sets up a convenient and efficient immune system in vitro which combines dendritic cell differentiation, allergens immune, mixed lymphocyte culture and so on. Finally the system successfully activates the proliferation of specific B cells and the secretion of a large number of specific IgE antibodies to shrimp allergen.
Background: We already know that abnormal immune cell infiltration is related to the pathogenesis of psoriasis (PSO), but the role of immune cell infiltration in the pathogenesis and treatment of psoriasis is unknown.The purpose of this study was to determine the diagnostic and predictive value of immune-related genes in psoriasis.Methods: Six gene expression datasets were downloaded from GEO database to analyze gene expression in psoriasis tissues. Single-sample gene set enrichment analysis (ssGSEA) was used to assess immune cell infiltration in psoriatic tissue. Immune-related genes were screened by overlapping immune-related genes with differentially expressed genes (DEGs). Protein interaction (PPI) networks are used to identify key DEGs. We analyzed the diagnostic value of key genes in psoriasis and the predictive value of key genes in three types of biological therapy. Immunohistochemistry (IHC) was used to detect gene expression in psoriasis tissues.Results: There were significant differences in immune cell infiltration between psoriatic tissue and normal skin tissue. PRC1, GATA3, IL1RN and CCL20 are the key genes of four subclusters in PPI network. PRC1, GATA3, IL1RN and CCL20 have diagnostic value in psoriasis, among which PRC1 and CCL20 have better diagnostic value than GATA3 and IL1RN. After treatment with effective biologic agents, the expression of PRC1, GATA3, IL1RN and CCL20 decreased in skin tissues of patients. GATA3 and IL1RN did not change significantly in patients with non-response to Ustekinumab, and the expression of GATA3 did not change significantly in patients with non-response to Etanercept, that showed good predictive value. Immunohistochemical analysis confirmed that the expression of PRC1, GATA3, IL1RN and CCL20 in PSO tissue was significantly different from that in normal skin tissue, and immune-related genes PRC1, GATA3, IL1RN and CCL20 could be used as auxiliary diagnostic indicators of psoriasis. GATA3 and IL1RN may serve as promising predictors of tumor necrosis factor inhibitors and IL-12/23 inhibitors therapy in patients.Conclusion: This study reveals the significance of immune genes in the pathogenesis and treatment of psoriasis. These key genes may provide new ideas for future treatments.
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