Men who have sex with men (MSM) have recently accounted for an alarmingly increasing proportion of HIV-1 transmission in China. In order to investigate the immune status as a result of CRF01_AE infection and CXCR4 co-receptor usage in a young Shanghai-based HIV-1-infected MSM population in Shanghai, 364 HIV-1-infected MSM with average age of 22.7 years old, newly diagnosed between Jan 2009 and Jul 2013 were analyzed for CD4+T cell count, subtyping using phylogenetic analysis, and viral co-receptor tropism using Geno2pheno and webPSSM in combination. A total of 276 individuals were identified as recently infected. Subtype assignment were as follows: 176 (63.8%) CRF01_AE, 77 (27.9%) CRF07_BC, and 23 (8.3%) subtype B. Besides, 24 second-generation recombinant strains were identified. A lower CD4+T cell count at baseline survey was observed among CRF01_AE strain-infected individuals, compared to those who were infected with CRF07_BC (P<0.01). The frequency of baseline CD4+T cell count <200 was higher and the frequency of CD4 T counts >500 lower in CRF01_AE infection than CRF07_BC infection. It is worth noting that 32.4%–40.9% of CRF01_AE strain-infected individuals were predicted to carry CXCR4-tropic viruses whereas none of CRF07_BC and subtype B were found to be as CXCR4-tropic viruses (P<0.001). As could be expected CXCR4 tropism was associated with lower CD4 T counts. This study revealed that CRF01_AE strains with high frequency of CXCR4 tropism are prevailing in the young MSM population in China and could potentially cause a severe loss of CD4+T cell count and rapid disease progression. A regular surveillance of HIV-1 subtypes, CD4+T cell count and viral co-receptor usage would be greatly beneficial for effectively monitoring disease progression, improvement of antiretroviral therapy strategy and prompt intervention of transmission.

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In agricultural vehicles with internal combustion engines, owing to the use of rear-wheel drive or four-wheel drive, it is difficult to obtain information regarding the slip of the driving wheels. Excessive wheel slip, an inevitable phenomenon occurring during agricultural activities, can easily damage the original soil surface and result in excessive energy consumption. To solve these problems, a distributed drive agricultural vehicle (DDAV) based on multi-information fusion was proposed. The actual travel reduction of each wheel was calculated by determining the vehicle parameters in order to deliver the required torque to the four drive wheels via sliding mode control (SMC) and incremental proportional-integral (PI) control. Through this process, the vehicle always operates in a straight line. Test results show that, on a uniform surface, the travel reduction of each wheel can be maintained at the target value by using the incremental PI control strategy, with only minor fluctuations, to make the vehicle run in a straight line (R2 = 0.9999). Furthermore, on a separated surface, the travel reduction of each wheel can be maintained at the target value, and using the SMC strategy enables more identical coefficient of gross tractions for each wheel to make the vehicle run in a straight line (R2 = 0.9902). Unlike the non-control strategy, the vehicle reaches a stable state within 1 s, owing to the use of a controller that can effectively reduce the impact of road changes on vehicle velocity. This study can provide a reference for the drive control of DDAVs.

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