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The amino acid permease (AAP) is an important transmembrane protein that is involved in the absorption and transport of amino acids in plants. We investigated the expression patterns of AtAAP genes in Arabidopsis thaliana, based on quantitative real-time PCR. The results revealed differential expression patterns of eight AtAAP genes in different tissues, with five genes (AtAAP1, AtAAP2, AtAAP6, AtAAP7, and AtAAP8) expressed at relatively high levels in both flowers and siliques, suggesting their shared functions in the accumulation of amino acids. In transgenic plants, with endosperm-specific overexpression of AtAAP1, both AtAAP1 and AtAAP6 were up-regulated in both the roots and siliques, while AtAAP2, AtAAP3, AtAAP4, and AtAAP5 showed similar expression levels in the stems and siliques, whereas AtAAP7 and AtAAP8 were expressed at their highest levels in the stems and roots. The results of the amino acid affinity experiments revealed varied absorption capacities for different amino acids, by AtAAP1, and increased acid amino contents in the reproductive organs. These results were verified in transgenic maize plants, with the overexpression of AtAAP1, revealing higher amino acid contents in the reproductive organs than those of the vegetative organs. Our study clearly demonstrated that the endosperm-specific promoter increased the amino acid contents in the reproductive organs and improved the effective utilization of organic nitrogen in plants.

This present study estimated the length-weight relationships of six fish species collected from the Xiaoshidao artificial reef zone, northern Yellow Sea. The samples were captured monthly from September 2020 to May 2021. During each sampling period, fish trap net (7 m, 20 mm mesh), consisting of 23 individual rectangular steel frames (22 × 35 cm) and one smaller-sized steel frame (19 × 30 cm) at each end which was deployed at random locations in the artificial reef zone for 24 h. The precision of measurement for the fish specimens is 0.1 cm total length and 0.01 g wet weight. Four new maximum lengths were recorded for S. hubbsi (20.5 cm), S. ommaturus (50.1 cm), Z. elongatus (43.5 cm) and P. fangi (20.0 cm).

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Large amounts of silt have been deposited on the seabed in China’s coastal areas due to intensive coastal development and marine raft aquaculture, which are the main causes of local marine environmental disasters. In this study, seabed silt was tested as a potential raw material for artificial reefs. The silt was mixed with cement in four proportions to create concrete specimens for use in silt artificial reefs (SARs). The compressive strength development and nutrient dissolution were examined in the SAR specimens. The hydration products of the SAR paste were investigated through X-ray diffraction (XRD), scanning election microscope (SEM), and differential scanning calorimetry (DSC) techniques. The results showed that the compression strength of the SAR specimens was inversely proportional to their seabed silt content. The SAR specimens were able to continuously dissolve nitrogen-containing nutrients. The presence of Ca(OH)2, commonly found in traditional concrete, was not detected, which may help improve the seaweed adhesion and biological effects of artificial reefs. The effective utilization of seabed silt could serve to restore and improve the marine ecological environment.

Artificial reefs (ARs) have been widely used to restore the seabed habitat and protect biodiversity. They can effectively increase the dissolved oxygen content in the bottom water layer by their disturbing effect of upwelling and downwelling. The bottom water is prone to hypoxia in summer due to the extreme weather of the global climate and excessive biomass in some marine ranching in northern China. Therefore, how to effectively use the upwelling effect of artificial reefs to alleviate this problem is a necessary subject of research. Generally, ARs are arranged by different intervals in a unit form on the seafloor, and the flow field effect is different from that of the individual reefs. However, few studies have been focused on the effect of layout mode on the flow field of a unit reef (UR). In this paper, we selected the interval between reefs (IR) and the angle of inflow (AI) as the influencing factors to study the flow field effect of UR. The upwelling and wake regions of 64 URs were presented by the efficiency and disturbance indices related to the flow characteristics and proposed an optimal layout mode having the best performance of the upwelling effect. The results showed that the interactions among the AI, the transverse, and longitudinal IRs were significant, and the AI has a significant influence on the flow field. These indices were effective and contribute to the layout optimization of UR. The AI close to 45° has a significant influence on the flow field effect of UR.