Specific recognition and separation of glycoproteins from complex biological solutions is very important in clinical diagnostics considering the close relationship between glycoproteins with the occurrence of diverse diseases, but the lack of materials with high selectivity and superior capture capacity still makes it a challenge. In this work, graphene oxide (GO) based molecularly imprinted polymers (MIPs) possessing double recognition abilities have been synthesized and applied as highly efficient adsorbents for glycoprotein recognition and separation. Boronic acid functionalized graphene oxide (GO-APBA) was first prepared and a template glycoprotein (ovalbumin, OVA) was then immobilized onto the surface of GO-APBA through boronate affinity. An imprinting layer was subsequently deposited onto GO-APBA surface by a sol-gel polymerization of organic silanes in aqueous solution. After the removal of the template glycoprotein, 3D cavities with double recognition abilities toward OVA were obtained in the as-prepared imprinted materials (GO-APBA/MIPs) because of the combination of boronate affinity and molecularly imprinted spatial matched cavities. The obtained GO-APBA/MIPs exhibited superior specific recognition toward OVA with imprinted factor (α) as high as 9.5, significantly higher than the corresponding value (4.0) of GO/MIPs without the introduction of boronic acid groups. Meanwhile, because of the synergetic effect of large surface area of graphene and surface imprinting, high binding capacity and fast adsorption/elution rate of GO-APBA/MIPs toward OVA were demonstrated and the saturation binding capacity of GO-APBA/MIPs could reach 278 mg/g within 40 min. The outstanding recognizing behavior (high adsorption capacity, highly specific recognition, and rapid binding rate) coupled to the facile and environmentally friendly preparation procedure makes GO-APBA/MIPs promising in the recognition, separation, and analysis of glycoproteins in clinics in the future.

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Chinese mitten crabs (Eriocheir sinensis) were collected after different periods of fattening under salinity (9‰): 0, 15, 30, 45 and 60 d, corresponding to the original phase (OP), initial phase (IP), middle phase (MP), late phase (LP) and final phase (FP) respectively. Biological indicators, proximate composition, free amino acids and flavour nucleotides were determined. The results show that the gonadosomatic index was increased for both genders during fattening, and the higher content of crude protein and lipid was found in IP and MP. The content of free amino acids and umami intensity were significantly higher in most edible tissues than in the overall samples. The contents of flavour nucleotides first increased with fattening time and then decreased in the later phases. However, in female crabs, the content of 5'-inosine monophosphate increased significantly in LP and FP compared with other phases. The findings indicate that fattening under salinity can increase gonadal index and umami intensity in E. sinensis.