As a significant Alzheimer’s
disease (AD)-specific biomarker,
the examination of brain-derived tau (BD-tau) is significant in terms
of the diagnosis and differentiation of neurological disorders. In
this research, sea urchin-like MoSe2 microspheres were
synthesized by hydrothermal synthesis, revealing significant SERS
activity, and the enhancement factor (EF) reached 1.44 × 107. The remarkable EF was mainly ascribed to the (i) charge
transfer between MoSe2 and MB, (ii) MB molecule’s
resonance effect, and (iii) molecular enrichment of porous structures.
Through an aptamer-antibody sandwich method, we prepared a BD-tau
SERS detection platform based on sea urchin-like MoSe2 microspheres.
The liner range was 0.05–200.00 pM with a limit of detection
(LOD) as low as 0.028 pM. Besides, the developed detection platform
demonstrated excellent selectivity and enabled early diagnosis of
AD patients. In general, the MoSe2 SERS detection platform
can well detect the concentration of BD-tau in mouse plasma samples,
showing great potential in specific recognition of AD among neurodegenerative
diseases.
The naphthalene derivative fluorescent probe F6 was synthesized and a 1 × 10−3 mol/L solution of Al3+ and other metals to be tested was prepared for the subsequent experiments. The Al3+ fluorescence system of the naphthalene derivative fluorescent probe F6 was successfully constructed as demonstrated by fluorescence emission spectroscopy. The optimal time, temperature and pH of the reaction were investigated. The selectivity and anti-interference ability of the probe F6 for Al3+ were investigated by fluorescence spectroscopy in a methanol solution. The experiments showed that the probe has high selectivity and anti-interference ability for Al3+. The binding ratio of F6 to Al3+ was 2:1, and the binding constant was calculated to be 1.598 × 105 M−1. The possible mechanism of the binding of the two was speculated. Different concentrations of Al3+ were added to Panax Quinquefolium and Paeoniae Radix Alba. The results showed that the recoveries of Al3+ were 99.75–100.56% and 98.67–99.67%, respectively. The detection limit was 8.73 × 10−8 mol/L. The experiments demonstrated that the formed fluorescence system can be successfully adapted for the determination of Al3+ content in two Chinese herbal medicines, which has good practical application.
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