In the present work, irregular Cu nanoparticle-decorated boron−carbon−nitrogen (Cu−BCN) nanosheets were successfully synthesized. A Cu−BCN dispersion was deposited on a bare glassy carbon electrode (GCE) to prepare an electrochemical sensor (Cu−BCN/GCE) for the detection of chloramphenicol (CAP) in the environment. Cu−BCN was characterized using high-resolution scanning transmission electron microscopy (HRSTEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) analysis, and X-ray photoelectron spectroscopy (XPS). The performance of the Cu−BCN/GCE was studied using electrochemical impedance spectroscopy (EIS), and its advantages were proven by electrode comparison. Differential pulse voltammetry (DPV) was used to optimize the experimental conditions, including the amount of Cu−BCN deposited, enrichment potential, deposition time, and pH of the electrolyte. A linear relationship between the CAP concentration and current response was obtained under the optimized experimental conditions, with a wide linear range and a limit of detection (LOD) of 2.41 nmol/L. Cu−BCN/GCE exhibited high stability, reproducibility, and repeatability. In the presence of various organic and inorganic species, the influence of the Cu−BCN-based sensor on the current response of CAP was less than 5%. Notably, the prepared sensor exhibited excellent performance in real-water samples, with satisfactory recovery.
Purpose Environmental conditions have an important impact on soil microbial communities. This study aimed to explore the effects of special management of bamboo wine brewing on the soil fungal community structure and dominant flora in Phyllostachys edulis (moso bamboo) forest. Methods In this experiment, the geometric center of moso bamboo forest was taken as the core, and three kinds of soils including the core area of bamboo forest (J1), non-bamboo forest area (J2) and marginal area of bamboo forest (J3) were selected as the research objects.The community structure and diversity of soil fungi and FunGuild function prediction were analyzed by high-throughput sequencing on IonS5TMXL sequencing platform using ITS sequencing technology. Results The results showed that: (1) in contrast to other bamboo forests and woodlands, the abundance of Ascomycetes in bamboo forest soil was higher than that of Basidiomycetes under bamboo wine making environment. (2) In the core area of bamboo forest J1, the soil fungal community complexity and species diversity were higher because of this special management. As samples gradually approached the core area of bamboo forest J1 from the non-bamboo forest J2, the dominant species of Basidiomycetes transitioned from Saitozyma podzolica to Burgoa anomala. Meanwhile, the dominant species of the Ascomycota shifted from Gliocladiopsis forsbergii to Fusarium oxysporum and Cyberlindnera saturnus. (3) The special bamboo forest management of bamboo wine brewing could reduce the abundance of plant pathogens and animal pathogens in bamboo forest soil, and Saitozyma and Penicillium, which had antibacterial effects, were important. As a result, the abundance of Fungal parasite-Undefinded Saprotroph (26.86%), Ectomycorrhizal (10.46%) and Ericoid Mycorrhizal (6.66%) increased significantly. Conclusions Our study indicated that the special management of bamboo wine brewing could change soil fungal community structure and diversity by increasing Basidiomycete abundance and inhibiting soil pathogens in moso bamboo forest.
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