Chemo-resistance is a huge obstacle encountered in the osteosarcoma (OS) treatment. Protein-coding mRNAs, as well as non-coding RNAs (ncRNAs), including long ncRNA (lncRNA), circular RNA (circRNA), and microRNA (miRNA), have been demonstrated to play an essential role in the regulation of cancer biology. However, the comprehensive expression profile and competing endogenous RNA (ceRNA) regulatory network between mRNAs and ncRNAs in the OS chemo-resistance still remain unclear. In the current study, we developed whole-transcriptome sequencing (RNA sequencing [RNAseq]) in the three paired multi-drug chemo-resistant and chemo-sensitive OS cell lines to comprehensively identify differentially expressed lncRNAs, circRNAs, miRNAs, and mRNAs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed for mRNAs with significantly different expression. Then the ceRNA networks combining lncRNAs, circRNAs, miRNAs, and mRNAs were predicted and constructed on the basis of the authoritative miRanda and TargetScan databases combined with the widely accepted vital drug resistance-related genes and signal transduction pathways. In addition, two constructed ceRNA regulatory pathways, lncRNAMEG3/hsa-miR-200b-3p/ AKT2 and hsa_circ_0001258/hsa-miR-744-3p/GSTM2, were randomly selected and validated by real-time qPCR, RNA immunoprecipitation (RIP), RNA pull-down assay, and dual luciferase reporter gene system. Taken together, our findings may provide new evidence for the underlying mechanism of OS chemo-resistance and uncover some novel targets for reversing it.Recently, competing endogenous RNA (ceRNA) networks have been reported as an important mechanism to explain a post-transcriptional

BackgroundAccording to the 2011 Infusion Nursing Standards of Practice, the low pH of intravenous vancomycin requires that it be administered through a central line. However, a careful review of the literature and a retrospective analysis of the experience at New York Hospital Queens (NYHQ) did not support the position of the Standards.PurposeA prospective, controlled, randomized clinical trial was conducted to determine if intravenous vancomycin could be safely administered through a novel midline catheter (POWERWAND®, Access Scientific, San Diego, CA).MethodsPatients scheduled to receive short-term (<6 days) intravenous vancomycin were randomly assigned to receive treatment through either a peripherally inserted central catheter (PICC) or the midline study device. Complications and the costs of insertion were recorded.ResultsThe two groups did not differ significantly with respect to total complications (17.9% with PICCs vs. 19.9% with the midline), phlebitis (0% vs. 0%) or thrombosis (0% vs. 0%). One suspected catheter-associated bloodstream infection did occur in the PICC group. Insertion costs were $90.00 less per insertion in the midline group.ConclusionsShort-term intravenous vancomycin can be safely and cost-efficiently administered in the deep vessels of the upper arm using the midline study device.

Highlights d Secondary bile acids reduce severity of experimental autoimmine uveitis (EAU) in mice d Altered bile acids are correlated with gut microbiota composition in EAU mice d Deoxycholic acid regulates the function of DCs via the TGR5-cAMP-PKA pathway

Previous work on the hydrocracking and hydroisomerization of alkanes over metal-impregnated anion-modified zirconium oxides (AZOs) is extended to long-chain alkanes, from n-heptane to high molecular weight polyolefins, using ZrO2 modified by anion-derived groups such as SO4 and WO3 and promoted with hydrogenation metals such as Pt or Ni. Depending on reaction temperature and time, high yields of C5−C12 isoalkanes or a mixture of gases with high selectivities to isobutane and isopentane can be produced. The products do not contain olefins, aromatics, or alkanes of carbon number higher than the feed. The iso/normal ratios of the alkane products obtained are significantly higher than those predicted by isomerization equilibria at the reaction conditions. It appears that higher (C7+) alkane hydrocracking over metal-promoted AZOs may not proceed via the conventional bifunctional mechanism involving initial dehydrogenation to an olefinic intermediate. The AZOs did not sinter or agglomerate during the hydrocracking reactions as indicated by particle size measurements. AZOs containing WO3 are more stable than those containing SO4, retaining their anionic groups in reactions at severe reducing conditions [300+ °C, 500−1200 psig (cold) H2]. XANES analysis of the Pt/ZrO2/WO3 catalyst indicated that both Pt and W maintained their zerovalent (Pt0) and hexavalent (W6+) states, respectively, during alkane hydrocracking as well as during recalcination in air.