The aim of this study was to study the regulation of abdominal fat deposition by DL- α -tocopherol acetate (vitamin E) in broilers. Diets supplemented with 50 IU vitamin E significantly diminished abdominal fat deposition in broilers at day 35. Transcriptome sequencing results for abdominal fat tissues of the control (FC) and 50 IU vitamin E-supplemented (FT) groups identified 602 differentially expressed genes (DEGs), which were enriched in cellular process, cell and cell part, and binding Gene Ontology terms. Pathway functional analysis revealed that the DEGs were enriched in 42 metabolic pathways. Notably, the most enriched pathway, fatty acid biosynthesis, was found to play a key role in lipid metabolism. Further, the key regulators of lipid metabolism, including fatty acid synthase, acetyl-CoA carboxylase alpha, and acyl-CoA synthetase long-chain family member 1, demonstrated decreased expression following vitamin E supplementation. Herein, we have identified pathways and genes regulated by vitamin E, thereby providing novel insights into the nutrients regulating abdominal fat deposition in broilers.
Variable transmission ratio racks show great potential in rice transplanters as a key component of variable transmission ratio steering to balance steering portability and sensitivity. The objective of this study was to develop a novel geometrical design method to achieve quick, high-quality modeling of the free curvilinear tooth profile of a variable transmission ratio rack. First, a discrete envelope motion 3D model was established between the pinion-sector and the variable transmission ratio rack blank based on the mapping relationship between the rotation angle of the pinion-sector and the displacement of the rack, according to the variable transmission ratio function. Based on the loop Boolean subtraction operation, which removed the pinion-sector from the rack blank during all moments of the discrete motion process, the final complex changing tooth shape of the variable transmission ratio rack was enveloped. Then, since Boolean cutting residues made the variable ratio tooth surface fluctuant and eventually affected the precision of the model, this study proposed a modification method for establishing a smooth and continuous tooth profile. First, a novel fitting algorithm used approximate variable ratio tooth profile points extracted from the Boolean cutting marks and generated a series of variable ratio tooth profiles by utilizing B-spline with different orders. Next, based on a transmission stability simulation, the variable ratio tooth profile with optimal dynamic performance was selected as the final design. Finally, tests contrasting the transmission stability of the machining samples of the initial variable ratio tooth profile and the final variable ratio tooth profile were conducted. The results indicated that the final variable ratio tooth profile is more effective than the initial variable ratio tooth profile. Therefore, the proposed variable ratio tooth profile modeling and modification method for eliminating Boolean cutting residues and improving surface accuracy is proved to be feasible.
Variable transmission ratio gears have shown considerable potential as key components of variable speed transmission steering boxes that balance steering portability and sensitivity. The objective of this study is to develop a novel method for designing variable transmission ratio tooth surfaces to circumvent the shortcomings of the meshing theory method. The involute rack of the variable transmission ratio pinion-and-rack pair is considered as a set of countless and infinitely close transverse sections, each of which is referred to as a gear element. Assuming evenly distributed circles in the physical domain of the variable transmission ratio tooth surfaces of the pinion, the problem of generating a tooth surface point is transformed into solving the specific geometric feature intersection point of each circle and corresponding gear element during variable transmission ratio meshing. Thereafter, a mathematical model and algorithm are developed to generate tooth surface points in an approximately even pattern. The normal deviations of the gear-element–generated tooth surface points and the corresponding tooth surface points calculated using meshing theory show that the proposed design method has a sufficiently high accuracy. Finite element models are employed analyzing the contact pattern, contact stress, bending stress, and transmission ratio error. The results indicate that the layout of approximately even tooth surface points using the gear element method is beneficial for improving the fitting precision of tooth surfaces and reducing the contact stress and transmission ratio error. Further, the same derived law of the flank and fillet tooth surfaces is conducive to the continuity of the tooth surfaces, which contributes toward reducing the bending stress. Finally, a prototype is manufactured via CNC end milling. The major contributions lie in a robust and efficient modeling method for variable transmission ratio tooth surfaces, which combined forms a solid foundation for their application.
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