Enrofloxacin (ENR) and sulfachloropyridazine combined with trimethoprim (TMP) were commonly used in poultries to treat bacterial infections. In this study, the pharmacokinetics of these antibiotics in four tissues of Taihe black‐boned silky fowls was studied. The results showed that these drugs were absorbed and distributed rapidly, with the highest concentration showing in skin. Meanwhile, ENR and its metabolite ciprofloxacin (CIP) and TMP were depleted slowly, particularly in skin with the elimination half‐lives being 37.1, 36.9, and 72.7 days, respectively. It may be attributed to the abundance of melanin in skin. The dietary risk assessment suggested that the long‐term dietary intakes of ENR, CIP, and TMP showed a considerable threat to human health. Based on the experiment, the withdrawal times of 284 days for ENR + CIP and 159 days for TMP were acquired, which showed that these drugs are not appropriate for the application in Taihe black‐boned silky fowls.

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Azo-linked porous organic polymers (APOPs) were synthesized by a simple azo reaction, with 2,2'-bis(trifluoromethyl)benzidine and 1,3,5-trihydroxybenzene as the monomers. The preparation process was mild, green and environmental-friendly, avoiding the use of high temperature, metal catalysis and harmful organic reagent. The obtained APOPs were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, powder X-ray diffraction and Brunauer-Emmett-Teller. With the prepared APOPs as adsorbent, a method of pipette tip solid-phase extraction-liquid chromatography tandem mass spectrometry detection (PTSPE-LC-MS/MS) was proposed for the analysis of target sedatives in animal tissues. Furthermore, the parameters for the extraction of five sedatives, including the amount of adsorbent, pH value, ion strength, elution solvent and volume, were investigated. Under the conditions optimized, the linear dynamic range was found from 0.1 µg kg− 1 to 10.0 µg kg− 1, and the limits of detection were ranged from 0.02 µg kg− 1 to 0.1 µg kg− 1. The method was assessed by the analysis of target sedatives in animal tissues, and the recoveries for the spiked pork samples and egg samples were 84–102% and 83–101%, respectively. The results show that the developed method of PTSPE-LC-MS/MS with APOPs as adsorbent is efficient for the analysis of trace sedatives in animal tissues.