In this study, bovine collagen hydrolysate was purified
via a series
of chromatograms, and the peptides with the highest activity for promoting
myoblast proliferation were identified by LC-MS-MS. It was demonstrated
that the peptide GDAGPPGPAGPAGPPGPIG (hydroxylation) could promote C2C12 proliferation (+18.5% ±
0.04, P < 0.05). The certain peptide was capable
of regulating the myogenic cell cycle and inhibiting myogenic cell
apoptosis. By combining molecular docking, quantitative real-time
PCR, and metabonomics, we suggested that the peptide GDAGPPGPAGPAGPPGPIG (hydroxylation)
might bind to FGFR1 and affect the expression of genes downstream
of FGFR1 and influence protein synthesis to promote myoblast proliferation.
The above results showed that the peptides isolated in this study
have the potential to alleviate sarcopenia in the elderly.
Gelatin is one of the essential materials for tissue engineering for its unique physicochemical properties. The endotoxin in gelatin needs to be controlled at a reasonable level to guarantee biosafety when applied to the human body. However, the isothermal titration calorimetry results showed specific interactions between gelatin and endotoxin, suggesting the difficulties of removing endotoxin from gelatin in mild conditions. In this work, a polyethyleneimine-grafted silica gel adsorbent for endotoxin was designed and prepared. Results showed that the prepared endotoxin adsorbent could remove endotoxin from gelatin without significantly changing the structures and properties of gelatin.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.