High temperature inhibits wheat grain filling. Polyamines (PAs) are closely associated with plant resistance caused by abiotic stress. However, little is known about the effect of PAs on the grain filling of wheat under heat stress. Two wheat varieties differing in heat resistance were used, and endogenous PAs levels were measured during grain filling under normal growth conditions outside the greenhouse (CK), artificially simulated high temperature (HT), artificially simulated high temperature plus exogenous application of spermine (HT + Spm) and artificially simulated high temperature plus spermidine (HT + Spd) treatments. Additionally, the variation of antioxidant enzymatic activities and osmotic adjustable substances content in grains was measured during grain filling. The results showed that compared with HT,HT + Spm and HT + Spd significantly increased grain weight of XC 6 (heat-resistant variety) by 19% and 5%, and XC 31 (heat-sensitive variety) by 31% and 34%, activity of superoxide dismutase (SOD), peroxidase (POD)and catalase (CAT) and content of Spm, Spd, and proline (Pro) increased significantly, while putrescine (Put), malondialdehyde (MDA) and soluble sugar (SS)contentdecreased during grain filling; The correlation analysis showed that grain weight was negatively correlated with the content of PUT, MDA, Pro and activity of SOD and CAT and positively correlated with the content of Spd and activity of POD in grains. Our results indicated that exogenous Spm and Spd could alleviate the heat injury of grain filling.
Two spring wheat varieties were used to study alleviating effects of exogenous spermine (Spm) and spermidine (Spd) on agronomic traits and photosynthesis under high temperature treatment (HT). Our results showed that HT significantly decreased grain mass per panicle of heat-resistant variety (XC 6) by 25% and heat-sensitive variety (XC 31) by 32%. After HT, i.e., at 13 d after flowering, the decrease in net photosynthetic rate of XC 6 (38%) was lower than that of XC 31 (53%); the reason for this was related to XC 6, which could maintain correspondingly normal chlorophyll content (Chl), stomatal conductance (gs), transpiration rate (E), and intercellular CO2 concentration. Exogenous Spm and Spd could increase relative water content, Chl, gs, E, the maximal quantum yield of PSII photochemistry, effective quantum yield of PSII photochemistry, antenna conversion efficiency, and photochemical quenching coefficient of flag leaves under HT. Our results indicated that the heat resistance of XC 6 is better than that of XC 31 and exogenous Spm and Spd could alleviate the heat injury of photosynthesis of wheat flag leaves. (normal growth conditions outside the greenhouse); DAF -days after flowering; DM -dry mass; E -transpiration rate; FM -fresh mass; Fv/Fm -maximal quantum yield of PSII photochemistry; Fv'/Fm' -antenna conversion efficiency; GMP -grain mass per panicle; GNP -grain number per panicle; gs -stomatal conductance; HT -artificially simulated high temperature; HT + Spd -artificially simulated high temperature + exogenous application spermidine (1 mM L -1 ); HT + Spm -artificially simulated high temperature + exogenous application of spermine (1 mM L -1 ); MDA -malondialdehyde; PAs -polyamines; PN -net photosynthetic rate; qp -photochemical quenching coefficient; RWC -relative water content; Spd -spermidine; Spm -spermine; TKM -thousand kernel mass; TM -turgid mass; TW -test mass; ФPSII -effective quantum yield of PSII photochemistry.
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