In this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These agents were used to extract the residual proteins in the non-aged and thermal aged samples. The protein extraction efficiencies of different extracting agents were quantitatively determined by bicinchoninic acid (BCA) method, and then processed by multivariate analysis of variance (MANOVA). The impact of the extraction process on the protein structure was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet absorption spectrum (UV) and circular dichroism (CD). The results showed that, for both non-aged and aged samples, the extraction efficiency of 2 M guanidine hydrochloride (GuHCl) was significantly higher than the other five agents, with less damage to the protein structure during the extraction process.
the current Web manifests the problem of information overload due to the success of the Web 2.0 paradigm in which users can provide new contents quickly. To help users find the most valuable information, a recommendation system is designed in which we use Euclidean formula to calculate the distance and Cosine formula to calculate the angle to distinguish between different kinds of users. Thus, similar users will receive related items. In the beginning, we will face some problems such as the cold start due to a small amount of data. We will advance some theories to solve the problem. With the proposed method, we can improve the quality of recommendation so that users can find the most valuable information.
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