ABSTRACT. In vitro grown cabbage (Brassica oleracea var. capitata) seedlings exposed to excess molybdenum (Mo) ions exhibited severely reduced plant growth at the cotyledonary stage. Adding 80 mM proline (Pro) to the Mo-treated medium could help 50% seedlings to overcome the toxicity and grow true leaves. Under excess Mo stress, seedlings accumulated blue/purple anthocyanin in their cotyledons and hypocotyls. The anthocyanin content under Mo with 40 mM Pro was 4-fold higher than the control medium, MS with 40 mM Pro. The presence of Pro in the excess-Mo condition reduced chlorophyll a, whereas the chlorophyll b content was much higher than the control media of MS with and without Pro. Moreover, supplementing various concentrations of Pro into the Mo-stressed condition promoted the seedlings with higher antioxidant enzyme activities of superoxide dismutase, ascorbate peroxidate, and catalase. In addition, genes in the anthocyanin biosynthesis and accumulation pathways, phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), flavonone 3-hydroxylase (F3H), leucoanthocyanidin dioxygenase (LDOX), and glutathione-S-transferase (GST), were all upregulated. Our study indicated that, under excess Mo stress, the antioxidant activity of cabbage seedlings was induced in an attempt to protect plants from the Mo-induced toxicity and exacerbated growth. Pro, on the other hand, functioned in producing higher antioxidant enzyme activity to partially help recover plant growth.
The selection of pioneer species is one of the key technologies for ecological restoration in Karst Rocky desertification areas. The purpose of this study is to explore the relationship between antioxidant enzyme activities of different pioneer plants in Karst Rocky desertification areas and their living conditions, environmental adaptability and other ecological factors, so as to screen out excellent species with good ecological and economic benefits and provide a theoretical basis for ecological restoration in Karst Rocky desertification areas. In this study, the pioneer plant samples were collected from the karst area of the Northwest Guangxi and their SOD, POD, CAT, GSH, APX and other antioxidant enzymes activities were determined and compared. Our results showed that the antioxidant enzyme activities of different plants were significantly various due to the influence of themselves and various external factors. The plant species with high antioxidant enzyme activities often had better soil ecological restoration effect. The activity of antioxidant enzymes in pioneer plants was closely related to soil organic matter, soil enzyme activity and other physical and chemical indexes. Antioxidant enzyme activity of plants in Karst regions may be a critical indicator for species selection for ecological restoration of Karst Rocky desertification.
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