Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production and how transcriptional regulations of these traits are interconnected. In-depth analysis of TFs involved in sexual development revealed that mutations causing defects in perithecia development frequently affect multiple other phenotypes, and the TFs associated with sexual development tend to be highly conserved in the fungal kingdom. Besides providing many new insights into understanding the function of F. graminearum TFs, this mutant library and phenome will be a valuable resource for characterizing the gene expression network in this fungus and serve as a reference for studying how different fungi have evolved to control various cellular processes at the transcriptional level.

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In this paper, we present an accurate method of moments (MoM) solution of the combined field integral equation (CFIE) using the multilevel fast multipole algorithm (MLFMA) for scattering by large, three-dimensional (3-D), arbitrarily shaped, homogeneous objects. We first investigate several different MoM formulations of CFIE and propose a new formulation, which is both accurate and free of interior resonances. We then employ MLFMA to significantly reduce the memory requirement and computational complexity of the MoM solution. Numerical results are presented to demonstrate the accuracy and capability of the proposed method. The method can be extended in a straightforward manner to scatterers composed of different homogeneous dielectric and conducting objects.

SummaryZearalenone (ZEA) is a polyketide mycotoxin produced by some species of Gibberella/Fusarium and causes hyperestrogenic syndrome in animals. ZEA occurs naturally in cereals infected by Gibberella zeae in temperate regions and threatens animal health. In this study, we report on a set of genes that participate in the biosynthesis of ZEA in G. zeae . Focusing on the non-reducing polyketide synthase (PKS) genes of the G. zeae genome, we demonstrated that PKS13 is required for ZEA production. Subsequent analyses revealed that a continuous, 50 kb segment of DNA carrying PKS13 consisted of three additional open reading frames that were coexpressed as a cluster during the condition for ZEA biosynthesis. These genes, in addition to PKS13 , were essential for the ZEA biosynthesis. They include another PKS gene ( PKS4 ) encoding a fungal reducing PKS; zearalenone biosynthesis gene 1 ( ZEB1 ), which shows a high similarity to putative isoamyl alcohol oxidase genes; and ZEB2 whose deduced product carries a conserved, basic-region leucine zipper domain. ZEB1 is responsible for the chemical conversion of β β β β -zearalenonol ( β β β β -ZOL) to ZEA in the biosynthetic pathway, and ZEB2 controls transcription of the cluster members. Transcription of these genes was strongly influenced by different culture conditions such as nutrient starvations and ambient pH. Furthermore, the same set of genes regulated by ZEB2 was dramatically repressed in the transgenic G. zeae strain with the deletion of PKS13 or PKS4 but not in the ZEB1 deletion strain, suggesting that ZEA or β β β β -ZOL may be involved in transcriptional activation of the gene cluster required for ZEA biosynthesis in G. zeae . This is the first published report on the molecular characterization of genes required for ZEA biosynthesis.

Figure 4 Comparison of normalized power density calculated using w x Ž . the recursive T-matrix algorithm with 11 a Tessellated geometry Ž . showing the scattering for 45Њ slanted metallic rectangle b Polar Ž . plot showing the normalized power density vs. for geometry of a

An efficient marching-on-in-time (MOT) scheme is presented for solving electric, magnetic, and combined field integral equations pertinent to the analysis of transient electromagnetic scattering from perfectly conducting surfaces residing in an unbounded homogenous medium. The proposed scheme is the extension of the frequency-domain adaptive integral/pre-corrected fast-Fourier transform (FFT) method to the time domain. Fields on the scatterer that are produced by space-time sources residing on its surface are computed: 1) by locally projecting, for each time step, all sources onto a uniform auxiliary grid that encases the scatterer; 2) by computing everywhere on this grid the transient fields produced by the resulting auxiliary sources via global, multilevel/blocked, space-time FFTs; 3) by locally interpolating these fields back onto the scatterer surface. As this procedure is inaccurate when source and observer points reside close to each other; and 4) near fields are computed classically, albeit (pre-)corrected, for errors introduced through the use of global FFTs. The proposed scheme has a computational complexity and memory requirement of ( log 2 ) and ( 3 2 ) when applied to quasiplanar structures, and of ( 3 2 log 2 ) and ( 2 ) when used to analyze scattering from general surfaces. Here, and denote the number of spatial and temporal degrees of freedom of the surface current density. These computational cost and memory requirements are contrasted to those of classical MOT solvers, which scale as ( 2 ) and ( 2 ), respectively. A parallel implementation of the scheme on a distributed-memory computer cluster that uses the message-passing interface is described. Simulation results demonstrate the accuracy, efficiency, and the parallel performance of the implementation.

Mycelia of Gibberella zeae (anamorph, Fusarium graminearum), an important pathogen of cereal crops, are yellow to tan with white to carmine red margins. We isolated genes encoding the following two proteins that are required for aurofusarin biosynthesis from G. zeae: a type I polyketide synthase (PKS) and a putative laccase. Screening of insertional mutants of G. zeae, which were generated by using a restriction enzyme-mediated integration procedure, resulted in the isolation of mutant S4B3076, which is a pigment mutant. In a sexual cross of the mutant with a strain with normal pigmentation, the pigment mutation was linked to the inserted vector. The vector insertion site in S4B3076 was a HindIII site 38 bp upstream from an open reading frame (ORF) on contig 1.116 in the F. graminearum genome database. The ORF, designated Gip1 (for Gibberella zeae pigment mutation 1), encodes a putative laccase. A 30-kb region surrounding the insertion site and Gip1 contains 10 additional ORFs, including a putative ORF identified as PKS12 whose product exhibits about 40% amino acid identity to the products of type I fungal PKS genes, which are involved in pigment biosynthesis. Targeted gene deletion and complementation analyses confirmed that both Gip1 and PKS12 are required for aurofusarin production in G. zeae. This information is the first information concerning the biosynthesis of these pigments by G. zeae and could help in studies of their toxicity in domesticated animals.